Redefining aberrant P53 expression of gastric cancer and its distinct clinical significance among molecular-histologic subtypes

医学 微卫星不稳定性 免疫组织化学 错义突变 移码突变 癌症 亚型 病理 肿瘤科 内科学 癌症研究 突变 生物 基因 遗传学 微卫星 等位基因 程序设计语言 计算机科学
作者
Shih‐Chiang Huang,Ian Yi‐Feng Chang,Tse-Ching Chen,Hsiao‐Ching Lin,Ching‐Chou Tsai,Jun‐Te Hsu,Chun‐Nan Yeh,Shih‐Cheng Chang,Ta‐Sen Yeh
出处
期刊:Asian Journal of Surgery [Elsevier BV]
标识
DOI:10.1016/j.asjsur.2024.05.121
摘要

Numerous studies have demonstrated a correlation between p53 overexpression and diminished survival in gastric cancer patients. However, conflicting findings exist, and we hypothesize that these discrepancies arise from the cancer's complexity and heterogeneity, coupled with a lack of consensus on aberrant p53 expression. We enrolled a cohort of 187 patients with surgically resected gastric cancer. Patient categorization was based on Epstein–Barr virus (EBV), microsatellite instability (MSI), and Lauren classification (intestinal, diffuse and mixed). Utilizing an incremental algorithm, we evaluated p53 immunohistochemical (IHC) patterns in all 187 cases, while next-generation sequencing was successfully performed on 152 cases to identify TP53 mutations (mutTP53). MutTP53 was identified in 32 % of the 152 cases, comprising 36 missense, 5 nonsense, and 7 frameshift alterations. Missense mutations predominantly correlated with p53 overexpression, while nonsense and frameshifting alterations related to null expression. Trial calculations indicated that null expression and a p53 IHC cutoff at >40 % offered the best prediction of mutTP53 (kappa coefficient, 0.427), with the highest agreement (0.524) observed in diffuse type and the lowest (0.269) in intestinal type. Null expression and a p53 IHC cutoff at >10 %, but not mutTP53 per se, provided the optimal prediction of survival outcome (p = 0.043), particularly in diffuse type (p = 0.044). Multivariate analysis showed that aberrant p53 IHC expression was not an independent prognostic factor. P53 IHC patterns are predictive biomarkers for mutTP53 and gastric cancer outcomes, where a prerequisite involves a nuanced approach considering cutoff values and molecular-histologic subtyping.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
沈彬彬完成签到,获得积分10
刚刚
czh完成签到,获得积分10
1秒前
小马甲应助YXH采纳,获得10
1秒前
2秒前
jayjiao完成签到,获得积分10
2秒前
2秒前
搜集达人应助留白采纳,获得10
2秒前
斯文莺发布了新的文献求助10
2秒前
whl完成签到 ,获得积分10
3秒前
余俊兰发布了新的文献求助10
3秒前
4秒前
少夫人完成签到,获得积分10
5秒前
zwj003完成签到,获得积分0
6秒前
英俊的铭应助Yun采纳,获得10
7秒前
7秒前
lalaland完成签到,获得积分10
7秒前
8秒前
8秒前
鹿立轩完成签到,获得积分10
10秒前
11秒前
Shawn完成签到,获得积分10
11秒前
小蘑菇应助哈哈哈采纳,获得10
11秒前
12秒前
13秒前
李爱国应助恋雅颖月采纳,获得10
13秒前
14秒前
留白发布了新的文献求助10
14秒前
fdkufghkd完成签到,获得积分10
17秒前
18秒前
18秒前
懵懂的幻桃完成签到 ,获得积分10
18秒前
flyfish完成签到,获得积分10
19秒前
19秒前
上官若男应助斯文莺采纳,获得30
20秒前
21秒前
21秒前
22秒前
Yun发布了新的文献求助10
22秒前
22秒前
22秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988786
求助须知:如何正确求助?哪些是违规求助? 3531116
关于积分的说明 11252493
捐赠科研通 3269766
什么是DOI,文献DOI怎么找? 1804771
邀请新用户注册赠送积分活动 881870
科研通“疑难数据库(出版商)”最低求助积分说明 809021