睡眠剥夺
海马体
莫里斯水上航行任务
腹腔注射
水迷宫
海马结构
心理学
神经科学
巴恩斯迷宫
麻醉
医学
内分泌学
内科学
认知
空间学习
作者
Yi-Ning Yan,John P. Williams,Kun Niu,Wenhao Zhang,Jianfeng Zhang,Le Shi,Jian-Xiong An
出处
期刊:PubMed
日期:2022-08-01
卷期号:25 (8): 980-988
标识
DOI:10.22038/ijbms.2022.63994.14112
摘要
Sleep deprivation is a common health problem in modern society and is negatively associated with deleterious effects on cognitive functions such as learning and memory ability. This study was undertaken to provide a detailed account of the effect of chronic ozone intraperitoneal injection on the deleterious effects of sleep deprivation on brain function in rats.Sleep deprivation was induced using the modified multiple platform model. The rats received REM sleep deprivation with an intraperitoneal injection of ozone or midazolam for 28 days. The effects of ozone on REM sleep deprivation-induced hippocampus-dependent learning and memory deficits were studied by the following approaches: Morris water maze (MWM) tests were used to evaluate spatial learning and memory of rats. Morphological changes in the brain were evaluated using hematoxylin and eosin (HE) staining. RNA-sequence was performed to seek a common mechanism. The expression of the targeted gene was examined by qPCR and Western blotting.Ozone intraperitoneal injection reversed spatial learning and memory deficits associated with REM sleep deprivation, ameliorating pathological brain damage and down-regulating the hippocampal expression of Sema3A in rats after REM sleep deprivation.Ozone intraperitoneal injection alleviated sleep deprivation-induced spatial learning and memory deficits by protecting hippocampal neurons via down-regulation of the expression of Sema3A in the hippocampus.
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