已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Analysis of Camellia oleifera transcriptome reveals key pathways and hub genes involved during different photoperiods

生物 转录组 油茶 钥匙(锁) 基因 植物 生物技术 遗传学 基因表达 生态学
作者
Jindong Yan,Jiacheng He,Jianan Li,Shuangshuang Ren,Ying Wang,Junqin Zhou,Xiaofeng Tan
出处
期刊:BMC Plant Biology [BioMed Central]
卷期号:22 (1) 被引量:7
标识
DOI:10.1186/s12870-022-03798-0
摘要

Camellia oleifera Abel. (C. oleifera) is an important traditional woody species in China that produces edible oil. However, the current literature lacks a proper understanding of C. oleifera's ability to adapt to different photoperiods.Our results indicate that the photoperiod can significantly impact flowering time in C. oleifera. We grew a total of nine samples under the short day condition (SD), middle day condition (MD) and long day condition (LD). Transcriptome analysis yielded 66.94 Gb of high-quality clean reads, with an average of over 6.73 Gb of reads for per sample. Following assembly, a total of 120,080 transcripts were obtained and 94,979 unigenes annotated. A total of 3475 differentially expressed genes (DEGs) were identified between the SD_MD, SD_LD, and MD_LD gene sets. Moreover, WGCNA identified ten gene modules. Genes in pink module (92 genes) were positively correlated with SD, and negatively correlated with both MD and LD. Genes in the magenta module (42 genes) were positively correlated with MD and negatively correlated with both LD and SD. Finally, genes in the yellow module (1758 genes) were positively correlated with both SD and MD, but negatively correlated with LD. KEGG enrichment analysis revealed that genes in the pink, magenta, and yellow modules were involved in flavonoid biosynthesis, amino sugar and nucleotide sugar metabolism and circadian rhythm pathways. Additionally, eight hub genes (GI, AP2, WRKY65, SCR, SHR, PHR1, ERF106, and SCL3) were obtained through network analysis. The hub genes had high connectivity with other photoperiod-sensitive DEGs. The expression levels of hub genes were verified by qRT-PCR analysis.An increase in light duration promotes earlier flowering of C. oleifera. Flavonoid biosynthesis, amino sugar and nucleotide sugar metabolism, and circadian rhythm pathways may function in the photoperiodic flowering pathway of C. oleifera. We also identified eight hub genes that may play a role in this pathway. Ultimately, this work contributes to our understanding of the photoperiodic flowering pathway of C. oleifera and further informs molecular breeding programs on the plant's photoperiodic sensitivity.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
wanci应助调皮秋采纳,获得10
2秒前
3秒前
酷波er应助zzz采纳,获得10
4秒前
斐嘿嘿发布了新的文献求助10
5秒前
Johnny发布了新的文献求助30
5秒前
十四发布了新的文献求助10
7秒前
Cruffin完成签到 ,获得积分10
8秒前
zephyr完成签到,获得积分10
8秒前
9秒前
负责怀莲发布了新的文献求助10
9秒前
义气芷蝶完成签到 ,获得积分10
13秒前
yaling完成签到,获得积分10
14秒前
zzz发布了新的文献求助10
15秒前
15秒前
苗条世德完成签到,获得积分10
16秒前
怕孤独的元风完成签到,获得积分10
16秒前
Owen应助feng采纳,获得10
17秒前
斐嘿嘿完成签到,获得积分10
17秒前
18秒前
万能图书馆应助十四采纳,获得10
18秒前
Newt发布了新的文献求助10
19秒前
20秒前
oldblack完成签到,获得积分10
21秒前
调皮秋发布了新的文献求助10
24秒前
飞舞伤寒完成签到,获得积分10
24秒前
英姑应助自由的秋灵采纳,获得10
26秒前
34秒前
34秒前
调皮秋完成签到,获得积分20
37秒前
爱宝乐宝福宝应助赵杰采纳,获得10
38秒前
40秒前
41秒前
47秒前
47秒前
田様应助科研通管家采纳,获得10
47秒前
猪猪hero应助科研通管家采纳,获得10
47秒前
领导范儿应助科研通管家采纳,获得10
47秒前
wanci应助科研通管家采纳,获得10
47秒前
在水一方应助科研通管家采纳,获得10
47秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989857
求助须知:如何正确求助?哪些是违规求助? 3531994
关于积分的说明 11255679
捐赠科研通 3270758
什么是DOI,文献DOI怎么找? 1805053
邀请新用户注册赠送积分活动 882195
科研通“疑难数据库(出版商)”最低求助积分说明 809208