Overexpression of the RNA binding protein MhYTP1 in transgenic apple enhances drought tolerance and WUE by improving ABA level under drought condition

生物 耐旱性 蒸腾作用 砧木 转基因 转基因作物 非生物成分 光合作用 栽培 用水效率 非生物胁迫 植物 基因 园艺 遗传学 生态学
作者
Tianli Guo,Na Wang,Yangchun Xue,Qingmei Guan,Steve van Nocker,Changhai Liu,Fengwang Ma
出处
期刊:Plant Science [Elsevier]
卷期号:280: 397-407 被引量:48
标识
DOI:10.1016/j.plantsci.2018.11.018
摘要

MhYTP1 is involved in post-transcriptional regulation as a member of YT521-homology (YTH) domain-containing RNA-binding proteins. We previously cloned MhYTP1 and found it participated in various biotic and abiotic stress responses. However, its function in long-term moderate drought has not been verified. Thus, we explored its biological role in response to drought. Under drought condition, the net photosynthesis rate (Pn) and water use efficiency (WUE) were significantly elevated in MhYTP1-overexpressing (OE) apple plants when compared with the non-transgenic (NT) controls. Further analysis indicated MhYTP1 expression was associated with elevated ABA content, increased stomatal density and reduced stomatal aperture. In addition, to gain insight into the function of stem-specific expression of MhYTP1, grafting experiments were performed. Interestingly, lower transpiration rate (Tr) and higher WUE were observed when transgenic plants were used as scions as opposed to rootstocks and when transgenic rather than NT plants were used as rootstocks, indicating MhYTP1 plays crucial roles in grafted plants. These results define a function for MhYTP1 in promoting tolerance to drought conditions, and suggest that MhYTP1 can serve as a candidate gene for future apple drought resistance breeding with the help of biotechnology.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
lili完成签到,获得积分10
2秒前
2秒前
yar应助卓梨采纳,获得10
2秒前
2秒前
打打应助毕十三采纳,获得30
3秒前
腾腾腾完成签到,获得积分10
4秒前
科目三应助Patty采纳,获得10
6秒前
腾腾腾发布了新的文献求助10
7秒前
如飘瑞雪发布了新的文献求助10
8秒前
无000发布了新的文献求助10
9秒前
lili发布了新的文献求助10
9秒前
中和皇极应助zxh123采纳,获得10
10秒前
11秒前
12秒前
好丽友完成签到,获得积分10
14秒前
18秒前
Patty发布了新的文献求助10
19秒前
19秒前
幽壑之潜蛟应助Moihan采纳,获得10
21秒前
Valky发布了新的文献求助10
22秒前
做一颗快乐的蛋完成签到,获得积分10
24秒前
25秒前
饿之巨人完成签到,获得积分10
25秒前
25秒前
啊啊啊发布了新的文献求助10
26秒前
卓梨发布了新的文献求助10
26秒前
26秒前
29秒前
30秒前
搞怪惜梦发布了新的文献求助10
31秒前
科研通AI2S应助如飘瑞雪采纳,获得10
31秒前
LienAo完成签到 ,获得积分10
33秒前
33秒前
酷波er应助搞怪惜梦采纳,获得10
35秒前
搜集达人应助畅快的易蓉采纳,获得10
36秒前
打打应助搞怪迎夏采纳,获得10
37秒前
37秒前
文艺谷蓝完成签到,获得积分10
37秒前
39秒前
高分求助中
Востребованный временем 2500
Agaricales of New Zealand 1: Pluteaceae - Entolomataceae 1040
지식생태학: 생태학, 죽은 지식을 깨우다 600
海南省蛇咬伤流行病学特征与预后影响因素分析 500
Neuromuscular and Electrodiagnostic Medicine Board Review 500
ランス多機能化技術による溶鋼脱ガス処理の高効率化の研究 500
Relativism, Conceptual Schemes, and Categorical Frameworks 500
热门求助领域 (近24小时)
化学 医学 材料科学 生物 工程类 有机化学 生物化学 纳米技术 内科学 物理 化学工程 计算机科学 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 电极
热门帖子
关注 科研通微信公众号,转发送积分 3462689
求助须知:如何正确求助?哪些是违规求助? 3056214
关于积分的说明 9050947
捐赠科研通 2745844
什么是DOI,文献DOI怎么找? 1506601
科研通“疑难数据库(出版商)”最低求助积分说明 696181
邀请新用户注册赠送积分活动 695693