体内
癌症研究
免疫系统
免疫疗法
细胞
化学
体外
生物相容性
自然杀伤细胞
氧化铁纳米粒子
癌细胞
细胞凋亡
细胞毒性
纳米技术
免疫学
癌症
材料科学
生物
纳米颗粒
生物化学
有机化学
生物技术
遗传学
作者
Liya Wu,Fuqiang Zhang,Zhenhong Wei,Xiuying Li,Hong Zhao,Huiying Lv,Rui Ge,He Ma,Hao Zhang,Bai Yang,Jing Li,Jinlan Jiang
出处
期刊:Biomaterials Science
[The Royal Society of Chemistry]
日期:2018-01-01
卷期号:6 (10): 2714-2725
被引量:99
摘要
Natural killer (NK)-cell-based immunotherapy has been reported to have promising prospects in the treatment of non-small cell lung cancer, one of the most common malignancies in the world. It has been proven that higher the NK cell infiltration into the tumor, the better is the curative effect. Therefore, it would be beneficial to develop a method that increases NK cell recruitment and infiltration into the tumor site. The purpose of this study was to establish an immune-cell delivery system for clear lung cancer cells based on magnetic nanoparticle (NP)-labeled NK cells that can be accumulated at the tumor site by placing a tiny external magnetic device inside animals. We developed superparamagnetic iron oxide NPs consisting of a magnetic Fe3O4 core and a shell of polydopamine (PDA) for magnetic targeting therapy. Fe3O4@PDA NPs possess favorable physiological stability and biocompatibility that facilitate their active uptake by NK cells. The biology of NK cells was not affected by the presence of NPs. In vitro and in vivo studies showed that Fe3O4@PDA NP-labeled NK cells significantly inhibited tumor growth and reduced the expression of Ki-67 and increased the apoptosis of A549 cancer cells. H&E staining showed Fe3O4@PDA NP-labeled NK cells, under a magnetic field, had higher intra-tumoral iron density and increased accumulation of CD56+ NK cells. Our results suggest that Fe3O4@PDA NPs are a promising magnetic nanomaterial that can manipulate immune cells, thereby inhibiting tumor growth.
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