陶氏病
适体
表位
化学
磷酸化
寡核苷酸
τ蛋白
高磷酸化
神经退行性变
DNA
细胞生物学
生物化学
计算生物学
分子生物学
生物
抗体
阿尔茨海默病
遗传学
疾病
病理
医学
作者
I-Ting Teng,Xiaowei Li,Hamad Yadikar,Zhihui Yang,Long Li,Yifan Lyu,Xiaoshu Pan,Kevin Wang,Weihong Tan
摘要
Tau proteins are proteins that stabilize microtubules, but their hyperphosphorylation can result in the formation of protein aggregates and, over time, neurodegeneration. This phenomenon, termed tauopathy, is pathologically involved in several neurodegenerative disorders. DNA aptamers are single-stranded oligonucleotides capable of specific binding to target molecules. Using tau epitopes predisposed for phosphorylation, we identified six distinct aptamers that bind to tau at two phosphorylatable epitopes (Thr-231 and Ser-202) and to full-length Tau441 proteins with nanomolar affinity. In addition, several of these aptamers also inhibit tau phosphorylation (IT4, IT5, IT6) and tau oligomerization (IT3, IT4, IT5, IT6). This is the first report to identify tau epitope-specific aptamers. Such tau aptamers can be used to detect tau in biofluids and uncover the mechanism of tauopathy. They can be further developed into novel therapeutic agents in mitigating tauopathy-associated neurodegenerative disorders.
科研通智能强力驱动
Strongly Powered by AbleSci AI