亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Hafnium oxide nanoparticle activated by radiotherapy to generate an anti-tumor immune response.

免疫系统 背向效应 免疫组织化学 医学 放射治疗 癌症研究 CD8型 病理 免疫疗法 免疫学 内科学
作者
Jérôme Galon,Marick Laé,Juliette Thariat,Sébastien Carrère,Zsuzsanna Pápai,M. Delannes,Paul Sargos,Philippe Rochaix,László Mangel,Fabienne Hermitte,Zoltán Szepes,Tamás Tornóczky,Isabelle Peyrottes,R. Tétreau,Marie-Christine Château,Sunyach Marie,Péter Ágoston,H. Brisse,Axel Le Cesne,Sylvie Bonvalot
出处
期刊:Journal of Clinical Oncology [Lippincott Williams & Wilkins]
卷期号:36 (15_suppl): e15149-e15149 被引量:2
标识
DOI:10.1200/jco.2018.36.15_suppl.e15149
摘要

e15149 Background: Radiotherapy (RT) can prime an anti-tumor immune response. Unfortunately, this response is not sufficient to allow tumor destruction and abscopal effect (effect on a distant untreated lesion) is hardly obtained. Hafnium oxide nanoparticle (HfO2-NP) is composed of high electron density material. After intratumoral injection (i.t.), HfO2-NPs are taken up by cancer cells and, when activated by RT, locally increase the radiation dose deposit, destroying more cancer cells than RT. We hypothesized that HfO2-NP + RT could enhance anti-tumor immune response, compared to RT alone, both in animal and human. Methods: Mouse CT26 cells were injected in both flanks of mice. When tumor volume is 50-120mm3, HfO2-NP (or vehicle) was injected by i.t. in the right flank tumors only, then irradiated (3x4Gy). Tumor growth was followed and animals sacrificed when a tumor reached 800mm3. Alternatively, tumors were collected 3 days after last RT fraction and immune cell infiltrates were analyzed by immunohistochemistry (IHC). Patients with locally advanced STS (NCT02379845) received either HfO2-NP + RT or RT alone. Pre- and post-treatment (biopsy and resection, respectively) tumor tissues for each patient are analyzed by immunohistochemistry and Digital Pathology for immune biomarkers and Pan-Immune gene expression profiling ( > 16 patients per arm). Results: In mouse, an abscopal effect was observed with HfO2-NP but not with RT alone. IHC analyses show that a significant increase of CD8+ cells is present in treated and untreated tumors, but no effect was observed for RT alone. IHC analysis (post- vs pre-treatment) show a marked increase of the biomarkers for patient treated with HfO2-NP + RT, particularly for CD8+ and PD1. No differences are seen for RT alone. The profile of gene expression HfO2-NP + RT differs from RT alone. Functional analysis of genes expression up-regulated in HfO2-NP + RT shows an increase of the cytokine and immune checkpoints expression, as T cell activation markers, compared to RT alone. Conclusions: HfO2-NP + RT bring marked changes to the tumor immune profile both in mouse model and in patients with STS, compared to RT. So far, these results show that HfO2-NP + RT induces a specific adaptive immune pattern. Clinical trial information: NCT02379845.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
xiao涂完成签到,获得积分10
17秒前
26秒前
闫雪发布了新的文献求助10
32秒前
打打应助闫雪采纳,获得10
43秒前
吗喽完成签到,获得积分10
47秒前
某某某完成签到,获得积分10
51秒前
1分钟前
爱吃橙子完成签到 ,获得积分10
1分钟前
丰富莹芝发布了新的文献求助10
1分钟前
思源应助丰富莹芝采纳,获得10
1分钟前
Akitten发布了新的文献求助10
1分钟前
qqq完成签到,获得积分10
1分钟前
1分钟前
小博发布了新的文献求助10
2分钟前
童大大完成签到,获得积分20
2分钟前
小博完成签到,获得积分10
2分钟前
CodeCraft应助糯糯采纳,获得10
3分钟前
汉堡包应助Plum22采纳,获得10
3分钟前
岁岁完成签到 ,获得积分10
4分钟前
4分钟前
Plum22发布了新的文献求助10
4分钟前
4分钟前
充电宝应助科研通管家采纳,获得10
4分钟前
cherlie应助Plum22采纳,获得20
5分钟前
5分钟前
5分钟前
5分钟前
6分钟前
6分钟前
6分钟前
6分钟前
丰富莹芝发布了新的文献求助10
6分钟前
6分钟前
糯糯发布了新的文献求助10
6分钟前
所所应助科研通管家采纳,获得10
6分钟前
科研通AI2S应助科研通管家采纳,获得10
6分钟前
喜悦幻灵完成签到 ,获得积分10
6分钟前
6分钟前
热心易绿完成签到 ,获得积分10
6分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990148
求助须知:如何正确求助?哪些是违规求助? 3532119
关于积分的说明 11256456
捐赠科研通 3271016
什么是DOI,文献DOI怎么找? 1805171
邀请新用户注册赠送积分活动 882288
科研通“疑难数据库(出版商)”最低求助积分说明 809228