生物膜
微塑料
基因组
微生物种群生物学
人类病原体
细菌
假单胞菌
微生物学
生物
生态学
基因
遗传学
作者
X. J. Wu,Jie Pan,Meng Li,Yao Li,Mark Bartlam,Li Wang
出处
期刊:Water Research
[Elsevier]
日期:2019-11-01
卷期号:165: 114979-114979
被引量:466
标识
DOI:10.1016/j.watres.2019.114979
摘要
Microplastics have been found to be ubiquitous in freshwater ecosystems, providing a novel substrate for biofilm formation. Here, we incubated biofilm on microplastics and two natural substrates (rock and leaf) under a controlled environment to investigate the differences of microbial community structure, antibiotic resistance gene (ARG) profiles, and ARG microbial hosts between biofilms on three types of substrates. Results from high-throughput sequencing of 16S rRNA gene revealed that microplastic biofilm had a distinctive community structure. Network analyses suggested that microplastic biofilm possessed the highest node connected community, but with lower average path length, network diameter and modularity compared with biofilm on two natural particles. Metagenomic analyses further revealed microplastic biofilm with broad-spectrum and distinctive resistome. Specifically, according to taxonomic annotation of ARG microbial hosts, two opportunisitic human pathogens (Pseudomonas monteilii, Pseudomonas mendocina) and one plant pathogen (Pseudomonas syringae) were detected only in the microplastic biofilm, but not in biofilms formed on natural substrates. Our findings suggest that microplastic is a novel microbial niche and may serve as a vector for ARGs and pathogens to new environment in river water, generating freshwater environmental risk and exerting adverse impacts on human health.
科研通智能强力驱动
Strongly Powered by AbleSci AI