In article number 1803730, Bettina V. Lotsch and co-workers develop an all-optical method to track analyte uptake and diffusion by a 2D nanosheet-based photonic multilayer system. Molecular diffusion of amines is monitored by a combination of optical spectroscopy and theoretical modelling, thus allowing the spatially resolved tracking of molecular diffusion in real time, based on a simple optical read-out.