Development of a near-infrared ratiometric fluorescent probe for glutathione using an intramolecular charge transfer signaling mechanism and its bioimaging application in living cells

A novel NIR ratiometric fluorescent probe HBT-GSH was developed for the selective detection of GSH over Cys and Hcy in aqueous solution. The signaling mechanism of the GSH-induced removal of an acetyl group and the switching on of ICT fluorescence was proposed.