柠檬黄单胞菌
柑橘溃疡病
效应器
生物
黄单胞菌
异位表达
溃疡
基因
发起人
柑橘×冬青
转录组
微生物学
基因表达
遗传学
植物
细菌
细胞生物学
园艺
橙色(颜色)
作者
Dongling Sun,Wei Rou,Yinghui Zhou,Tao Zhuo,Xiaojing Fan,Xun Hu,Huasong Zou
标识
DOI:10.1016/j.bbrc.2018.05.194
摘要
Xanthomonas citri subsp. citri (Xcc) is the causal agent of citrus canker, a serious bacterial disease that affects citrus trees worldwide. The ectopic expression of TAL effector AvrXa7 in Xcc suppressed canker development. The Xcc strain expressing avrXa7 induced a yellow symptom around the inoculation site. Transcriptome analysis revealed 315 differentially expressed genes, which were categorized into several functional groups. The more interesting genes were those involved in the biosynthesis of terpene and ethylene. In particular, the linoleate 13 S-lipoxygenase gene CsLOX2-1 was found to possess the AvrXa7 binding sequence in the promoter region. The recognition of AvrXa7 to the CsLOX2-1 promoter was subsequently confirmed by yeast one-hybrid and electrophoretic mobility shift experiments. This demonstrated that the TALE effector AvrXa7 promotes CsLOX2-1 expression by directly binding to the promoter sequence. Our findings contribute a valuable clue to identifying the potential genes that can be used to prevent citrus canker.
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