嵌合体(遗传学)
脂质体
化学
药物输送
细胞质
体外
融合蛋白
生物物理学
生物化学
细胞生物学
重组DNA
生物
基因
有机化学
作者
Atsushi Tabata,Yukimasa Ohkubo,Eriko Sakakura,Toshifumi Tomoyasu,Kazuto Ohkura,Hideaki Nagamune
出处
期刊:PubMed
日期:2012-06-01
卷期号:32 (6): 2323-9
被引量:10
摘要
Cholesterol-dependent cytolysins (CDCs) are pore-forming toxins from Gram-positive bacteria. The aim of this study was to investigate the potential of a CDC, intermedilysin, as a drug-delivery system (DDS) for clinical application.Intermedilysin was modified by the addition of a disulfide bridge to regulate pore formation, by swapping domain 4 to provide cholesterol-binding capacity, and by the introduction of a targeting domain. The resultant chimera protein, His-LTBP-CDC(ss)(IP), was investigated for its use as a DDS tool in vitro.His-LTBP-CDC(ss)(IP) exhibited a regulated pore-forming capacity under reducing conditions. This chimera protein was able to deliver a drug-carrier liposome specifically to the target cell, to be endocytosed into the cell with subsequent release of the components into the cytoplasm.A chimera protein derived from the bacterial pore-forming toxin intermedilysin (His-LTBP-CDC(ss)(IP)) forms the basis for a novel DDS tool.
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