DNAJB4 identified as a potential breast cancer marker: evidence from bioinformatics analysis and basic experiments

乳腺癌 医学 基因 基因表达 癌症 生存分析 生物信息学 癌症研究 发病机制 疾病 肿瘤科 生物 内科学 遗传学
作者
Linlong Mo,Jiayou Liu,Ziquan Yang,Xun Gong,Fanlun Meng,Rongyang Zou,Lingmi Hou,Fang Fang
出处
期刊:Gland surgery [AME Publishing Company]
卷期号:9 (6): 1955-1972 被引量:9
标识
DOI:10.21037/gs-20-431
摘要

Breast cancer (BC) is the leading cause of tumor-related death in women worldwide, but its pathogenesis is not clear. The efficient screening of new therapeutic targets for BC through bioinformatics and biological experimental techniques has become a hot topic in BC research.The bioinformatics method was used to analyze the gene chips and obtain the hub genes, playing an important role in the development of BC. The biological processes (BP) involved in the hub genes were analyzed by Bingo, and the impact of each hub gene on disease-free survival (DFS) and overall survival (OS) in BC patients was evaluated in the Kaplan-Meier Plotter database. The expression of DNAJB4, the hub gene with the greatest degree and having an effect on the prognosis of BC patients, was detected in BC cell lines and clinicopathological specimens. And DNAJB4 was selected for further biological experiments and clinical prognosis verification.Ten hub genes including DNAJB4, the greatest degree genes, were found by bioinformatics analysis of BC gene chips. DNAJB4 expressions in both BC cell lines and clinicopathological specimens were detected and the results showed that DNAJB4 was significantly down-regulated in BC cell lines and tissues. After interfering with the expression of DNAJB4, it was found that the invasion and migration ability of MDA-MB-231 cell line was significantly enhanced in vitro. The clinical survival data of BC patients showed that patients with high DNAJB4 expression had longer DFS.DNAJB4 may be a tumor suppressor gene in BC as it could regulate invasion and migration of BC cells and its expression level is related to the prognosis of BC patients. Nevertheless, further researches are still necessary to verify its role in BC so as to provide evidences for clinical guidance regarding diagnosis and treatment.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
英姑应助开花的五花肉采纳,获得10
刚刚
pskgg完成签到,获得积分10
刚刚
gujiamin完成签到,获得积分10
刚刚
刚刚
KY Mr.WANG完成签到,获得积分10
1秒前
banana95完成签到 ,获得积分10
1秒前
accept完成签到,获得积分10
2秒前
Marksman497发布了新的文献求助10
2秒前
2秒前
Jasper应助Mia采纳,获得10
2秒前
jackhlj完成签到,获得积分10
3秒前
huanhuan发布了新的文献求助10
3秒前
宇文数学完成签到 ,获得积分10
5秒前
5秒前
oyxz发布了新的文献求助10
6秒前
暮夏七发布了新的文献求助10
6秒前
Owen应助zhouleiwang采纳,获得10
8秒前
9秒前
没烦恼发布了新的文献求助10
9秒前
10秒前
XIAOMUMU发布了新的文献求助10
12秒前
NexusExplorer应助huanhuan采纳,获得10
12秒前
12秒前
动听以晴发布了新的文献求助10
12秒前
13秒前
李爱国应助狂暴的蜗牛0713采纳,获得10
13秒前
14秒前
14秒前
linlinlinlin发布了新的文献求助10
16秒前
oyxz完成签到,获得积分10
17秒前
韩十四完成签到,获得积分10
17秒前
17秒前
Shirley完成签到,获得积分10
18秒前
18秒前
开花的五花肉完成签到,获得积分10
19秒前
斯文败类应助哲999采纳,获得10
20秒前
阿林琳琳发布了新的文献求助10
20秒前
Rez发布了新的文献求助10
20秒前
爆米花应助巫凝天采纳,获得30
22秒前
aj完成签到,获得积分10
23秒前
高分求助中
Evolution 10000
Sustainability in Tides Chemistry 2800
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
A new species of Velataspis (Hemiptera Coccoidea Diaspididae) from tea in Assam 500
Diagnostic immunohistochemistry : theranostic and genomic applications 6th Edition 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3156078
求助须知:如何正确求助?哪些是违规求助? 2807458
关于积分的说明 7873196
捐赠科研通 2465782
什么是DOI,文献DOI怎么找? 1312412
科研通“疑难数据库(出版商)”最低求助积分说明 630102
版权声明 601905