体内
生物发光成像
四斯潘宁
体内分布
荧光素酶
细胞外小泡
生物发光
临床前影像学
细胞生物学
微泡
体外
分子成像
微泡
生物
化学
计算生物学
细胞
细胞培养
生物化学
生物技术
基因
小RNA
遗传学
转染
作者
Dhanu Gupta,Xiuming Liang,С. И. Павлова,Oscar P. B. Wiklander,Giulia Corso,Ying Zhao,Osama Saher,Jeremy Bost,Antje M. Zickler,András Piffkó,Cécile L. Maire,Franz Ricklefs,Oskar Gustafsson,Virginia Castilla‐Llorente,Manuela O. Gustafsson,Rakibe Beklem Bostancıoğlu,Doste R. Mamand,Daniel W. Hagey,André Görgens,Joel Z. Nordin,Samir El Andaloussi
标识
DOI:10.1080/20013078.2020.1800222
摘要
Extracellular vesicles (EVs) are naturally occurring nano-sized carriers that are secreted by cells and facilitate cell-to-cell communication by their unique ability to transfer biologically active cargo. Despite the pronounced increase in our understanding of EVs over the last decade, from disease pathophysiology to therapeutic drug delivery, improved molecular tools to track their therapeutic delivery are still needed. Unfortunately, the present catalogue of tools utilised for EV labelling lacks sensitivity or are not sufficiently specific. Here, we have explored the bioluminescent labelling of EVs using different luciferase enzymes tethered to CD63 to achieve a highly sensitive system for in vitro and in vivo tracking of EVs. Using tetraspanin fusions to either NanoLuc or ThermoLuc permits performing highly sensitive in vivo quantification of EVs or real-time imaging, respectively, at low cost and in a semi-high throughput manner. We find that the in vivo distribution pattern of EVs is determined by the route of injection, but that different EV subpopulations display differences in biodistribution patterns. By applying this technology for real-time non-invasive in vivo imaging of EVs, we show that their distribution to different internal organs occurs just minutes after administration.
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