Luteolin Suppresses Sepsis-Induced Cold-Inducible RNA-Binding Protein Production and Lung Injury in Neonatal Mice

新生儿败血症 腹腔注射 促炎细胞因子 败血症 细胞因子 化学 木犀草素 下调和上调 炎症 医学 药理学 免疫学 男科 内科学 生物化学 类黄酮 抗氧化剂 基因
作者
Yuanyuan Zhang,Jia Zhang,Yifan Ren,Teng Li,Jianbin Bi,Zhaoqing Du,Rongqian Wu
出处
期刊:Shock [Ovid Technologies (Wolters Kluwer)]
卷期号:55 (2): 268-273 被引量:21
标识
DOI:10.1097/shk.0000000000001624
摘要

ABSTRACT Neonatal sepsis is a life-threatening inflammatory condition. Extracellular cold-inducible RNA-binding protein (CIRP), a proinflammatory mediator, plays a critical role in the pathogenesis of sepsis-induced lung injury in neonates. Luteolin, a polyphenolic flavonoid, has potent anti-inflammatory properties. However, the effects of luteolin on CIRP production and neonatal sepsis-induced lung injury remained unknown. We therefore hypothesize that treatment with luteolin suppresses CIRP production and attenuates lung injury in neonatal sepsis. To study this, sepsis was induced in C57BL/6J mouse pups (5–7 days) by intraperitoneal cecal slurry injection (CSI). One hour after CSI, luteolin (10 mg/kg body weight) or vehicle (normal saline) was administered through intraperitoneal injection. CIRP mRNA and protein were determined and lung injury was assessed at 10 h after CSI. Our results showed that administration of luteolin decreased CIRP mRNA and protein, improved lung architecture, reduced lung edema, and apoptosis after CSI. To examine the direct effect of luteolin on CIRP production, peritoneal macrophages were isolated from neonatal mice and stimulated with 100 ng/mL LPS with or without the presence of luteolin. The result indicates that luteolin directly inhibited LPS-induced CIRP production in neonatal macrophages. In addition, luteolin also downregulated hypoxia-inducible factor-1α (HIF-1α) and NOD-like receptor 3 (NLRP3) expression in septic neonates and in LPS-stimulated neonatal macrophages. In conclusion, administration of luteolin suppresses CIRP production and attenuates lung injury in neonatal sepsis. The beneficial effect of luteolin may be related to downregulation of HIF-1α and NLRP3 expression in neonatal macrophages. Luteolin may be developed as an adjunctive therapy for neonatal sepsis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
华仔应助舒适的紫丝采纳,获得10
刚刚
shilong.yang完成签到,获得积分10
1秒前
大个应助LLeaf采纳,获得30
1秒前
e任思完成签到,获得积分10
1秒前
酷波er应助甜蜜水蜜桃采纳,获得10
1秒前
ken完成签到,获得积分10
1秒前
1秒前
1秒前
snailanli完成签到 ,获得积分10
2秒前
野原新之助完成签到 ,获得积分10
2秒前
小王发布了新的文献求助10
3秒前
ljyx完成签到,获得积分10
3秒前
旧巷歌者发布了新的文献求助10
3秒前
liyue6090完成签到,获得积分20
4秒前
洛尘完成签到,获得积分10
5秒前
第二提琴手完成签到,获得积分10
6秒前
欢呼败完成签到,获得积分10
6秒前
Zhouzhou完成签到,获得积分10
6秒前
追三发布了新的文献求助10
6秒前
李健应助执笔客采纳,获得10
7秒前
萧梦旋完成签到,获得积分10
7秒前
ljyx发布了新的文献求助10
8秒前
阿怪12333发布了新的文献求助10
8秒前
8秒前
8秒前
陆靖易完成签到,获得积分10
9秒前
9秒前
ee关闭了ee文献求助
10秒前
科研通AI2S应助VDC采纳,获得10
10秒前
kkx发布了新的文献求助10
10秒前
11秒前
12秒前
WuYiHHH完成签到,获得积分10
12秒前
Ava应助ttg990720采纳,获得10
12秒前
笨笨志泽发布了新的文献求助10
12秒前
bkagyin应助樂酉采纳,获得10
13秒前
13秒前
你好完成签到,获得积分10
13秒前
ruiyingtong完成签到,获得积分10
14秒前
无花果应助大胆的如容采纳,获得10
14秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Conference Record, IAS Annual Meeting 1977 820
England and the Discovery of America, 1481-1620 600
Teaching language in context (Third edition) by Derewianka, Beverly; Jones, Pauline 550
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3582884
求助须知:如何正确求助?哪些是违规求助? 3152234
关于积分的说明 9491736
捐赠科研通 2854472
什么是DOI,文献DOI怎么找? 1569191
邀请新用户注册赠送积分活动 735033
科研通“疑难数据库(出版商)”最低求助积分说明 721006