Fluorescent Detection of Methyl Mercury in Aqueous Solution and Live Cells Using Fluorescent Probe and Micelle Systems

化学 检出限 甲基汞 荧光 Mercury(编程语言) 胶束 水溶液 水溶液中的金属离子 离子 无机化学 色谱法 环境化学 有机化学 物理 生物累积 量子力学 计算机科学 程序设计语言
作者
Semin Oh,Jongyong Jeon,Jaewook Jeong,Joohee Park,Eun‐Taex Oh,Heon Joo Park,Keun‐Hyeung Lee
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:92 (7): 4917-4925 被引量:79
标识
DOI:10.1021/acs.analchem.9b05025
摘要

It is highly challenging to develop fast and sensitive fluorescent methods for monitoring organic mercury in purely aqueous solutions as well as live cells. Especially, selective fluorescent detection of methylmercury over inorganic mercury ions has not been reported. We developed a fast and sensitive fluorescent detection method for Hg2+ ions as well as methylmercury using an amino acid–based fluorescent probe (1) and SDS micelles. The fluorescent probe in SDS micelles detected sensitively and selectively Hg2+ ions and methylmercury among 16 metal ions in purely aqueous solution by the enhancement of the red emission at 575 nm, and the detection of methylmercury was completed within 1 min. The probe in SDS micelles with EDTA showed highly sensitive and selective turn on detection for methylmercury over Hg2+. The limit of detection was 9.1 nM for Hg2+ (1.8 ppb, R2 = 0.989) and 206 nM for CH3Hg+ (R2 = 0.997). 1 rapidly penetrated live cells and detected intracellular Hg2+ ions as well as CH3Hg+ by the enhancement of both red emissions and green emissions. Subsequent treatment of EDTA into the cell confirmed the selective detection of methylmercury in the cells. The present work indicated that the fluorescent probe with micelle systems provided a fast, sensitive, and selective detection method for monitoring inorganic mercury as well as methyl mercury.
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