Effects of the prebiotic inulin‐type fructans on post‐antibiotic reconstitution of the gut microbiome

Journal of Applied MicrobiologyVolume 130, Issue 3 p. 634-649 Original Article Effects of the prebiotic inulin-type fructans on post-antibiotic reconstitution of the gut microbiome C. Tao, Corresponding Author taochangli@126.com orcid.org/0000-0003-0398-9646 Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China Correspondence Changli Tao and Hongwei Shao, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China. E-mail: taochangli@126.com; shaohw2000@163.comSearch for more papers by this authorW. Zeng, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorQ. Zhang, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorG. Liu, orcid.org/0000-0002-7100-9411 College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaSearch for more papers by this authorF. Wu, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorH. Shen, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorW. Zhang, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorH. Bo, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorH. Shao, Corresponding Author shaohw2000@163.com Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China Correspondence Changli Tao and Hongwei Shao, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China. E-mail: taochangli@126.com; shaohw2000@163.comSearch for more papers by this author C. Tao, Corresponding Author taochangli@126.com orcid.org/0000-0003-0398-9646 Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China Correspondence Changli Tao and Hongwei Shao, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China. E-mail: taochangli@126.com; shaohw2000@163.comSearch for more papers by this authorW. Zeng, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorQ. Zhang, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorG. Liu, orcid.org/0000-0002-7100-9411 College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, ChinaSearch for more papers by this authorF. Wu, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorH. Shen, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorW. Zhang, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorH. Bo, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, ChinaSearch for more papers by this authorH. Shao, Corresponding Author shaohw2000@163.com Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China Correspondence Changli Tao and Hongwei Shao, Guangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou 510006, China. E-mail: taochangli@126.com; shaohw2000@163.comSearch for more papers by this author First published: 19 August 2020 https://doi.org/10.1111/jam.14827Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Abstract Aims Interventions using prebiotic inulin-type fructans (ITFs) are widely prescribed to modulate the gut microbiota composition and activity to promote health. However, the impacts of ITFs on post-antibiotic reconstitution of the gut microbiome remain incompletely understood. The aim of the present study was to investigate the effects of ITFs supplementation on intestinal inflammation, the composition of the intestinal microbiota and the colonic transcriptome after antibiotic treatment. Methods and Results Male BALB/c mice were subjected to an antibiotic cocktail (ABx) treatment for 7 days, and their microbiomes were then reconstituted either spontaneously or with ITFs supplementation (5%) for 14 days. Our data showed that ITFs supplementation delayed the recovery of antibiotic-induced colitis compared with the spontaneous recovery. Neither ITFs supplementation nor spontaneous recovery could restore the microbial community composition at the genus level back to its initial composition. ITFs supplementation increased the relative abundance of some beneficial bacteria and butyrate levels, but resulted in selective blooms of some opportunistic pathogens and elevated the pathways associated with diseases linked to gut microbiota function. Both ITFs supplementation and spontaneous recovery could restore the colonic transcriptome nearly to the initial profile to a certain extent; however, ITFs supplementation delayed the restoration of the immunoglobulin genes compared to spontaneous recovery. Conclusion These data showed that post-antibiotic ITFs consumption did not always lead to beneficial effects but might lead to potential adverse effects in the context of dysbiosis. Significance and Impact of the Study These findings highlighted that caution is required when supplementing ITFs to restore intestinal homeostasis in the context of dysbiosis resulting from broad-spectrum antibiotics. Supporting Information Filename Description jam14827-sup-0001-Supinfo.docxWord document, 559.5 KB Table S1. Primers used for real-time qRT-PCR. Figure S1. PICRUSt results of the second-level KEGG pathways in the gut microbiome. Figure S2. GO functional analysis of the differentially expressed genes in the colon. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume130, Issue3March 2021Pages 634-649 RelatedInformation