烟酰胺单核苷酸
移植
男科
内分泌学
内科学
卵巢
卵巢组织冷冻保存
NAD+激酶
毛囊
生物
发情周期
卵泡
卵巢组织
不育
保持生育能力
生理学
医学
烟酰胺腺嘌呤二核苷酸
生育率
怀孕
生物化学
酶
遗传学
人口
环境卫生
作者
Michael J. Bertoldo,Valentina Rodriguez Paris,Debra A. Gook,Melissa C Edwards,Katherine Wu,Cai Liang,Maria B. Marinova,Lindsay E. Wu,Kirsty A. Walters,Robert B. Gilchrist
出处
期刊:Reproduction
[Bioscientifica]
日期:2021-02-01
卷期号:161 (2): 215-226
被引量:6
摘要
Ovarian tissue cryopreservation and future transplantation is the only strategy to preserve the fertility of young female adolescent and prepubertal patients. The primary challenge to ovarian graft longevity is the substantial loss of primordial follicles during the period of ischaemia post-transplantation. Nicotinamide mononucleotide (NMN), a precursor of the essential metabolite NAD + , is known to reduce ischaemic damage. Therefore, the objective of the current study was to assess the impact of short- and long-term NMN administration on follicle number and health following ovarian tissue transplantation. Hemi-ovaries from C57Bl6 mice ( n = 8–12/group) were transplanted under the kidney capsule of bilaterally ovariectomised severe combined immunodeficient (SCID) mice. Recipient mice were administered either normal drinking water or water supplemented with NMN (2 g/L) for either 14 or 56 days. At the end of each treatment period, ovarian transplants were collected. There was no effect of NMN on the resumption of oestrous or length of oestrous cycles. Transplantation significantly reduced the total number of follicles with the greatest impact observed at the primordial follicle stage. We report that NMN did not prevent this loss. While NMN did not significantly impact the proportion of apoptotic follicles, NMN normalised PCNA expression at the primordial and intermediate stages but not at later stages. In conclusion, NMN administration did not prevent ovarian follicle loss under the conditions of this study.
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