甲基化
DNA甲基化
生物
宫颈癌
宫颈上皮内瘤变
接收机工作特性
肿瘤科
癌症研究
癌症
内科学
医学
遗传学
基因
基因表达
作者
Stèfanie Dick,Lisanne Verhoef,Lise MA De Strooper,Iuliana Ciocănea‐Teodorescu,G. Bea A. Wisman,Chris J.L.M. Meijer,Maaike C.G. Bleeker,Renske D.M. Steenbergen,Daniëlle A.M. Heideman
出处
期刊:Epigenomics
[Future Medicine]
日期:2020-09-01
卷期号:12 (18): 1569-1578
被引量:20
标识
DOI:10.2217/epi-2019-0331
摘要
Aim: To evaluate the triage performance of six host-cell DNA methylation markers derived from two genome-wide discovery screens for detection of cervical precancer (cervical intraepithelial neoplasia 3 [CIN]) and cancer. Materials & methods: Human papillomavirus-positive cervical scrapes of controls (≤CIN1; n = 352) and women diagnosed with CIN3 (n = 175) or cervical cancer (n = 50) were analyzed for methylation of ASCL1, LHX8, ST6GALNAC5, GHSR, SST and ZIC1. Results: Methylation levels increased significantly with disease severity (all markers p < 0.001). Three markers (ASCL1, LHX8, ZIC1) showed receiver operating characteristic curves with area under the curve >0.800 after leave-one-out cross-validation. Bi-marker panel ASCL1/LHX8 had highest area under the curve (0.882), and detected 83.4% of CIN3 and all cervical cancers at specificity of 82.4%. Conclusion: All six methylation markers showed an equivalent, high performance for the triage of human papillomavirus-positive women using cervical scrapes with complementarity between markers.
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