Pesticide residues enter a lake through the water cycle, causing harm to the water environment and human health. It is necessary to select highly sensitive fluorescence spectroscopy to detect pesticides (bifenthrin, prochloraz, and cyromazine), and a support vector machine (SVM) is used to analyze the concentration of pesticides. In addition, this paper adopts K-fold cross validation and a grid search to optimize the SVM algorithm. The performance evaluation index and running time prove the reliability of the results of this experiment. They show that fluorescence spectroscopy combined with SVM is efficient in predicting pesticide residue content.