光电二极管
软件可移植性
多路复用
炸薯条
荧光
滤波器(信号处理)
材料科学
光学滤波器
发光二极管
镜头(地质)
计算机科学
计算机硬件
光电子学
光学
物理
生物信息学
电信
计算机视觉
生物
程序设计语言
作者
Sung-Hun Yun,Jisung Park,Seul-Bit-Na Koo,Chan-Young Park,Yu-Seop Kim,Jong-Dae Kim
出处
期刊:Sensors
[MDPI AG]
日期:2021-10-20
卷期号:21 (21): 6945-6945
被引量:7
摘要
The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera with embedded lens was used instead of photodiodes or an industrial camera. A compact filter wheel using a sliding tape is integrated, and the excitation LEDs are fixed at a 45° angle near the PCR chip, eliminating the need of additional filter wheels. The results show precise positioning of the filter wheel with an error less than 20 μm. Fluorescence detection results using a reference dye and standard DNA amplification showed comparable performance to that of the photodiode system.
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