安普克
肌发生
心肌细胞
生物
细胞生物学
信号转导
蛋白激酶A
基因表达
肌球蛋白
骨骼肌
尼泊尔卢比1
分子生物学
激酶
基因
生物化学
内分泌学
作者
Meng Xu,Xiaoling Chen,Zhiqing Huang,Daiwen Chen,Bing Yu,Jun He,Hong Chen,Jie Yu,Yuheng Luo,Ping Zheng
标识
DOI:10.1080/10495398.2021.1927065
摘要
In this study, our aim is to investigate the effect of dimer procyanidin B2 [epicatechin-(4β-8)-epicatechin] (PB2) on porcine skeletal myofiber gene expression in vitro. Our data showed PB2 promoted the protein expression of slow myosin heavy chain (MyHC) in porcine myotubes, concomitant with the increases in mRNA levels of MyHC I, MyHC IIa and Tnni1. We also found PB2 activated AMPK signaling in porcine myotubes. NRF1 and CaMKKβ that are two important upstream factors of AMPK, and Sirt1 and PGC-1α that are two major downstream factors of AMPK, were also up-regulated by PB2. The mechanism study showed the effect of PB2 on slow-twitch myofiber gene expression was abolished by AMPK inhibitor compound C or by AMPKα1 siRNA. Together, we found PB2 induced porcine skeletal slow-twitch myofiber gene expression by AMPK signaling pathway.
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