骨髓
免疫荧光
环钻
病理
人骨
抗原
染色
抗体
生物
医学
免疫学
体外
生物化学
作者
Francesca M. Panvini,Simone Pacini,Stefano Mazzoni,Simón Méndez‐Ferrer
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 119-126
被引量:2
标识
DOI:10.1007/978-1-0716-1425-9_10
摘要
Immunofluorescence is an indispensable method for the identification, localization and study of the expression of target antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections of human bone marrow. However, the procedure shows technical limitations because of the chemical and physical treatments required for sample processing before imaging. Here we describe a revisited protocol to obtain high-resolution images of human bone marrow trephine biopsies, improving the antigen-antibody recognition and preserving the morphology and the architecture of the bone marrow microenvironment.
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