Method of Using Raman Spectroscopy to Understand the Conformation of Fibrous Proteins

Raman spectroscopy has been widely used in the research of fibrous proteins because of the insensitivity to moisture, less amount of sample, and better signal-to-noise ratio. In recent years, Raman spectroscopy is adopted to investigate the secondary structures of solid or aqueous protein, the conformation transition under different conditions (concentration, temperature, pressure, pH, chemical modification, external force, etc.), the orientation of the molecular chains, and some important chemical bonds. Here, we will introduce the methods for using Raman spectroscopy to analyze the conformation and orientation of samples, which would be an efficient method to get the "structure-property" relationship.