Phytochemical investigation of the rhizomes of Paris polyphylla var. yunnanensis led to the isolation of four ecdysteroids (1−4) including two new ecdysteroids (1−2), and two known spirostane steroids (5−6). Their structures were elucidated by extensive spectroscopic analyses as well as comparisons with those reported in the literature. All the isolates were assayed for their immunosuppressive and adipogenesis inhibitory activities but were inactive.