脱硫弧菌
甲烷杆菌
环境化学
环境科学
化学
地质学
细菌
古细菌
古生物学
作者
Shiling Zheng,Meng Li,Yang Liu,Fanghua Liu
出处
期刊:Water Research
[Elsevier]
日期:2021-07-31
卷期号:202: 117490-117490
被引量:96
标识
DOI:10.1016/j.watres.2021.117490
摘要
Abstract Geobacter, as a typical electroactive microorganism, is the “engine” of interspecies electron transfer (IET) between microorganisms. However, it does not have a dominant position in all natural environments. It is not known what performs a similar function as Geobacter in coastal zones. Metagenomic and metatranscriptomic analysis revealed that Desulfovibrio and Methanobacterium species were the most abundant in electrochemically active aggregates. Metatranscriptomic analysis showed that Desulfovibrio species highly expressed genes for ethanol metabolism and extracellular electron transfer involving cytochromes, pili and flagella. Methanobacterium species in the aggregates also expressed genes for enzymes involved in reducing carbon dioxide to methane. Pure cultures demonstrated that the isolated Desulfovibrio sp. strain JY contributed to aggregate conductivity and directly transferred electrons to Methanothrix harundinacea, which is unable to use H2 or formate. Most importantly, further coculture studies indicated that Methanobacterium strain YSL might directly accept electrons from the Desulfovibrio strain JY for the reduction of carbon dioxide to methane in the aggregate. This finding suggested that the possibility of DIET by Desulfovibrio similar to Geobacter species in conductive methanogenic aggregates can not be excluded.
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