已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

3.88 Å structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

多面体 衣壳 核糖核酸 生物物理学 细胞质 低温电子显微 化学 结晶学 生物 细胞生物学 病毒 病毒学 生物化学 基因 重组DNA 夜蛾
作者
Xuekui Yu,Lei Jin,Zhen Zhou
出处
期刊:Nature [Springer Nature]
卷期号:453 (7193): 415-419 被引量:277
标识
DOI:10.1038/nature06893
摘要

Cytoplasmic polyhedrosis virus (CPV) is a member of a large family of double-stranded RNA viruses, but it is unique in having a single shell capsid yet being fully capable of cell entry and mRNA transcription. The structure of this virus has now been determined by single-particle cryo-electron microscopy (cryoEM) to a resolution of 3.88 Å. The technique allows the polypeptide backbone to be traced without the need to make a crystal. The high-resolution structure shows how conformational switching is exploited to make railroad-like 'sliding' tracks for RNA packing and transcription and reveals an mRNA releasing hole coupled with distinctive capping machinery. With this and several other recent publications, cryo-electron microscopy underlines its credentials as a system capable of atomic-resolution in structural studies. This paper presents a high resolution structure of the cytoplasmic polyhedroasis virus (CPV) obtained by single particle cryo electron microscopy, and shows that the polypeptide backbone can be traced without the need of making a crystal. Cytoplasmic polyhedrosis virus (CPV) is unique within the Reoviridae family in having a turreted single-layer capsid contained within polyhedrin inclusion bodies, yet being fully capable of cell entry and endogenous RNA transcription1,2,3,4. Biochemical data have shown that the amino-terminal 79 residues of the CPV turret protein (TP) is sufficient to bring CPV or engineered proteins into the polyhedrin matrix for micro-encapsulation5,6. Here we report the three-dimensional structure of CPV at 3.88 Å resolution using single-particle cryo-electron microscopy. Our map clearly shows the turns and deep grooves of α-helices, the strand separation in β-sheets, and densities for loops and many bulky side chains; thus permitting atomic model-building effort from cryo-electron microscopy maps. We observed a helix-to-β-hairpin conformational change between the two conformational states of the capsid shell protein in the region directly interacting with genomic RNA. We have also discovered a messenger RNA release hole coupled with the mRNA capping machinery unique to CPV. Furthermore, we have identified the polyhedrin-binding domain, a structure that has potential in nanobiotechnology applications.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
细腻的雅山完成签到 ,获得积分10
2秒前
3秒前
4秒前
欧皇完成签到,获得积分20
4秒前
5秒前
南方完成签到 ,获得积分10
5秒前
研友Zby14n发布了新的文献求助10
7秒前
皮咻完成签到 ,获得积分10
8秒前
zls完成签到 ,获得积分10
8秒前
何劲松完成签到,获得积分10
8秒前
娜娜完成签到 ,获得积分10
10秒前
韦老虎完成签到,获得积分10
12秒前
棉花糖猫弦完成签到 ,获得积分0
13秒前
高天雨完成签到 ,获得积分10
14秒前
H1998完成签到,获得积分10
16秒前
19秒前
19秒前
迷你的夜天完成签到 ,获得积分10
20秒前
大妙妙完成签到 ,获得积分10
21秒前
Jenlisa完成签到 ,获得积分10
21秒前
轻松元绿完成签到 ,获得积分10
23秒前
wu发布了新的文献求助10
24秒前
考博圣体完成签到 ,获得积分20
25秒前
H1998发布了新的文献求助10
25秒前
27秒前
求知若渴完成签到,获得积分10
27秒前
研友Zby14n完成签到 ,获得积分10
31秒前
wswswsws完成签到,获得积分10
31秒前
李爱国应助lzzj采纳,获得10
31秒前
海城好人完成签到,获得积分10
31秒前
32秒前
Always完成签到,获得积分10
34秒前
34秒前
乐乐乐乐乐乐应助H1998采纳,获得10
34秒前
帅气的小兔子完成签到 ,获得积分10
35秒前
研友Zby14n发布了新的文献求助10
36秒前
不焦虑完成签到,获得积分20
37秒前
打打应助ff采纳,获得10
39秒前
高高的笑柳完成签到 ,获得积分10
39秒前
assholechea发布了新的文献求助10
40秒前
高分求助中
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
麻省总医院内科手册(原著第8版) (美)马克S.萨巴蒂尼 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
宽禁带半导体紫外光电探测器 388
COSMETIC DERMATOLOGY & SKINCARE PRACTICE 388
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3142628
求助须知:如何正确求助?哪些是违规求助? 2793418
关于积分的说明 7806632
捐赠科研通 2449709
什么是DOI,文献DOI怎么找? 1303403
科研通“疑难数据库(出版商)”最低求助积分说明 626861
版权声明 601309