化学
毛细管电泳
电解质
离解(化学)
电泳
质子化
离子强度
电泳图谱
离解常数
阳离子聚合
色谱法
离子键合
分析物
限制
分析化学(期刊)
物理化学
水溶液
离子
有机化学
机械工程
受体
电极
工程类
生物化学
作者
Iva Zusková,Alena Novotná,Kateřina Včeláková,Bohuslav Gaš
标识
DOI:10.1016/j.jchromb.2006.03.015
摘要
The dependence of the effective electrophoretic mobility on pH of the background electrolyte was experimentally determined by capillary zone electrophoresis (CZE) for cationic forms of amino acids. The pH of the background electrolytes was in the highly acidic range, 1.6-2.6 pH units, to ensure a high degree of protonation of the amino acids. Poly(vinyl alcohol) was added to the background electrolytes to avoid possible adsorption of the analytes at the inner capillary wall. Non-linear regression of the experimental data was applied to obtain the parameters of the relevant regression functions--the actual mobilities and mixed dissociation constants corresponding to the actual ionic strength. The extended Onsager and Debye-Hückel law was used to calculate the limiting mobilities and thermodynamic dissociation constants. The comparison of the experimental electropherogram with the computer prediction by PeakMaster using the determined data is presented for the selected sample of amino acids.
科研通智能强力驱动
Strongly Powered by AbleSci AI