泰特
生物
激活剂(遗传学)
紫胶操纵子
发起人
四环素
抄写(语言学)
报告基因
基因
基因表达
酿酒酵母
分子生物学
酵母
细胞生物学
抑制因子
遗传学
抗生素
语言学
哲学
作者
Eloi Garí,Lídia Piedrafita,Martí Aldea,Enrique Herrero
出处
期刊:Yeast
[Wiley]
日期:1997-07-01
卷期号:13 (9): 837-848
被引量:577
标识
DOI:10.1002/(sici)1097-0061(199707)13:9<837::aid-yea145>3.0.co;2-t
摘要
A set of Saccharomyces cerevisiae expression vectors has been developed in which transcription is driven by a hybrid tetO-CYC1 promoter through the action of a tetR-VP16 (tTA) activator. Expression from the promoter is regulated by tetracycline or derivatives. Various modalities of promoter and activator are used in order to achieve different levels of maximal expression. In the presence of antibiotic in the growth medium at concentrations that do not affect cell growth, expression from the tetO promoter is negligible, and upon antibiotic removal induction ratios of up to 1000-fold are observed with a lacZ reporter system. With the strongest system, overexpression levels comparable with those observed with GAL1-driven promoters are reached. For each particular promoter/tTA combination, expression can be modulated by changing the tetracycline concentration in the growth medium. These vectors may be useful for the study of the function of essential genes in yeast, as well as for phenotypic analysis of genes in overexpression conditions, without restrictions imposed by growth medium composition. © 1997 by John Wiley & Sons, Ltd.
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