In VitroActivity of Protoanemonin, an Antifungal Agent

Protoanemonin, the lactone of γ-hydroxyvinylacrylic acid, isolated from Pulsatilla alpina has in vitro activity against fungi. The MIC is 15 µg/ml and RNA inhibition seems to be the first target of the drug. The LD50 of protoanemonin in male Swiss albino mice was 190 mg/kg.