病毒
密度梯度
色谱法
化学
蔗糖
甲型流感病毒
病毒学
转子(电动)
生物
生物化学
物理
量子力学
作者
D. J. S. Arora,Pierre Tremblay,Richard Bourgault,Serge Boileau
标识
DOI:10.1016/0003-2697(85)90103-4
摘要
A simple procedure which enables the concentration and purification of influenza virus, using an angular rotor, is described. Virus is concentrated over a sucrose step gradient. The same gradients are reused and volumes up to 4 liters are concentrated in 1 day. The concentrated virus is further purified by a simplified density-gradient technique. No host cell protein is detectable in the final product. The technique offers a broad application potential for concentrating and purifying other viruses.
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