生物
寡核苷酸
基因
遗传学
计算生物学
内含子
下垂
DNA
背景(考古学)
外显子
历史
古生物学
考古
作者
Sara S. Gaddis,Qi Wu,Howard D. Thames,John DiGiovanni,Earl F. Walborg,Michael C. MacLeod,Karen M. Vásquez
出处
期刊:Oligonucleotides
[Mary Ann Liebert, Inc.]
日期:2006-06-01
卷期号:16 (2): 196-201
被引量:49
标识
DOI:10.1089/oli.2006.16.196
摘要
Triplex technology offers a useful approach for site-specific modification of gene structure and function both in vitro and in vivo. Triplex-forming oligonucleotides (TFOs) bind to their target sites in duplex DNA, thereby forming triple-helical DNA structures via Hoogsteen hydrogen bonding. TFO binding has been demonstrated to site-specifically inhibit gene expression, enhance homologous recombination, induce mutation, inhibit protein binding, and direct DNA damage, thus providing a tool for gene-specific manipulation of DNA. We have developed a flexible web-based search engine to find and annotate TFO target sequences within the human and mouse genomes. Descriptive information about each site, including sequence context and gene region (intron, exon, or promoter), is provided. The engine assists the user in finding highly specific TFO target sequences by eliminating or flagging known repeat sequences and flagging overlapping genes. A convenient way to check for the uniqueness of a potential TFO binding site is provided via NCBI BLAST. The search engine may be accessed at spi.mdanderson.org/tfo
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