人参
多糖
生物测定
黄体期
孵化
化学
五加科
生物
传统医学
色谱法
生物化学
医学
激素
遗传学
病理
替代医学
作者
Leang‐Shin Wu,Sheh Yi Sheu,Chong Huang,Chien Chao Chiu,Jan Chi Huang,Jenn Rong Yang,Wai Xiong Lian,Chin-Hsing Lai,Yuh Pan Chen,Jen H. Lin
标识
DOI:10.1142/s0192415x0000043x
摘要
Our previous report first showed evidence that polysaccharides isolated from ginseng leaves obtained from Jilin, China possess luteotropic activities. In this study, we made further investigations on the root and flowers of Korean ginseng by means of the same bioassay system described briefly as follows. Frozen-thawed bovine luteal cells (1 x 10(5) cells/ml/well) in M199 were incubated in 24-well culture plates at 37 degrees C in a 5 % CO2 incubator. Ten microl of tested drugs with 1, 10 and 100 microg/ml were added into each well. After 4- and 24-hr incubation, the media were harvested and assayed for progesterone by an enzyme immunoassay. The production of progesterone from cells is the indicator for evaluating the action of tested drugs. Results showed that hot water extracts ofginseng flowers (GF-1) with 10 to 100 microg/ml significantly increased progesterone production, whereas those from ginseng root (GR-1) could not. Crude polysaccharides (GF-2) isolated from GF-1 is the active component and the small molecules (mw < 10,000 dalton) are excluded, indicating that the ginseng root has no luteotropic activities, but the polysaccharides of ginseng flowers have.
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