Effect of preparation method and cholesterol on drug encapsulation studies by phospholipid liposomes

脂质体 超声 小泡 化学 磷脂 挤压 色谱法 双层 脂质双层 药物输送 细胞色素c 生物物理学 生物化学 有机化学 材料科学 线粒体 生物 冶金
作者
Fatma Melis Cagdas,Nurettin Ertugral,Şeyda Bucak,N. Zeynep Atay
出处
期刊:Pharmaceutical Development and Technology [Taylor & Francis]
卷期号:16 (4): 408-414 被引量:21
标识
DOI:10.3109/10837451003774401
摘要

Unilamellar liposomes, prepared from synthetic lipid mixture of DMPC and DMPG either by sonication or extrusion, were used to entrap water soluble and water insoluble molecules to investigate the efficacy of encapsulation by different liposome preparation methods. In the case of entrapment of hydrophilic protein cytochrome-C, the solutions were subjected to a series of ultrafiltration steps to eliminate any free protein outside the vesicles. It was observed that the protein could be encapsulated by the vesicles only if cholesterol was present in the bilayer. The release of cytochrome-C was observed spectrophotometrically upon vesicle-breakdown. The amount of protein encapsulated depended on the method of preparation and was found to be 10 times greater in extruded liposomes compared to those produced by sonication. Hydrophobic Vitamin E, on the other hand, could be encapsulated in the liposome bilayer, independently of the presence of cholesterol and the method of preparation. These fundamental results can be used to develop more efficient drug encapsulations and to have better understanding about their release.
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