生物
细胞周期蛋白依赖激酶2
细胞周期蛋白依赖激酶1
黑腹果蝇
Cdc25型
遗传学
突变体
表型
细胞周期蛋白
细胞生物学
第1周
互补
细胞周期蛋白
突变
有丝分裂
细胞周期
基因
作者
Mary Ellen Lane,Marion Elend,Doris Heidmann,Anabel Herr,Sandra Marzodko,Alf Herzig,Christian F. Lehner
出处
期刊:Genetics
[Oxford University Press]
日期:2000-05-01
卷期号:155 (1): 233-244
被引量:51
标识
DOI:10.1093/genetics/155.1.233
摘要
Abstract In higher eukaryotes, cyclin E is thought to control the progression from G1 into S phase of the cell cycle by associating as a regulatory subunit with cdk2. To identify genes interacting with cyclin E, we have screened in Drosophila melanogaster for mutations that act as dominant modifiers of an eye phenotype caused by a Sevenless-CycE transgene that directs ectopic Cyclin E expression in postmitotic cells of eye imaginal disc and causes a rough eye phenotype in adult flies. The majority of the EMS-induced mutations that we have identified fall into four complementation groups corresponding to the genes split ends, dacapo, dE2F1, and Cdk2(Cdc2c). The Cdk2 mutations in combination with mutant Cdk2 transgenes have allowed us to address the regulatory significance of potential phosphorylation sites in Cdk2 (Thr 18 and Tyr 19). The corresponding sites in the closely related Cdk1 (Thr 14 and Tyr 15) are of crucial importance for regulation of the G2/M transition by myt1 and wee1 kinases and cdc25 phosphatases. In contrast, our results demonstrate that the equivalent sites in Cdk2 play no essential role.
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