参考基因
基因
抄写(语言学)
管家基因
实时聚合酶链反应
基因表达
生物
计算生物学
信使核糖核酸
分子生物学
RNA聚合酶
甘油醛3-磷酸脱氢酶
核糖核酸
遗传学
语言学
哲学
作者
Aleksandar Radonić,Stefanie Thulke,Ian M Mackay,Olfert Landt,W. Siegert,Andreas Nitsche
标识
DOI:10.1016/j.bbrc.2003.11.177
摘要
Today, quantitative real-time PCR is the method of choice for rapid and reliable quantification of mRNA transcription. However, for an exact comparison of mRNA transcription in different samples or tissues it is crucial to choose the appropriate reference gene. Recently glyceraldehyde 3-phosphate dehydrogenase and β-actin have been used for that purpose. However, it has been reported that these genes as well as alternatives, like rRNA genes, are unsuitable references, because their transcription is significantly regulated in various experimental settings and variable in different tissues. Therefore, quantitative real-time PCR was used to determine the mRNA transcription profiles of 13 putative reference genes, comparing their transcription in 16 different tissues and in CCRF-HSB-2 cells stimulated with 12-O-tetradecanoylphorbol-13-acetate and ionomycin. Our results show that “Classical” reference genes are indeed unsuitable, whereas the RNA polymerase II gene was the gene with the most constant expression in different tissues and following stimulation in CCRF-HSB-2 cells.
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