柚皮素
重组DNA
羟基化
拟南芥
大肠杆菌
生物化学
山奈酚
互补DNA
体外
生物
芹菜素
化学
酶
立体化学
类黄酮
基因
突变体
抗氧化剂
出处
期刊:Phytochemistry
[Elsevier]
日期:2002-07-01
卷期号:60 (6): 589-593
被引量:107
标识
DOI:10.1016/s0031-9422(02)00155-3
摘要
cDNA corresponding to a flavonol synthase gene from Arabidopsis thaliana was cloned and expressed in Escherichia coli. The recombinant protein was purified to near-homogeneity and the catalytic properties of the enzyme were studied in vitro. Together with kaempferol and apigenin the recombinant protein synthesised the (2R,3S)-cis- and (2S,3S)-trans-isomers of dihydrokaempferol from the (2S)- and (2R)-isomers of naringenin, respectively. Flavanones and dihydroflavanols differing in degree of A- or B-ring hydroxylation were also accepted as substrates.
科研通智能强力驱动
Strongly Powered by AbleSci AI