脂滴
细胞器
过氧化物酶体
脂质代谢
线粒体
细胞生物学
蛋白质-脂质相互作用
化学
酿酒酵母
生物
酵母
生物物理学
双分子荧光互补
生物化学
膜蛋白
膜
整体膜蛋白
基因
作者
Pu Jing,Cheol Woong Ha,Shuyan Zhang,Jong Pil Jung,Won‐Ki Huh,Pingsheng Liu
出处
期刊:Protein & Cell
[Springer Nature]
日期:2011-06-01
卷期号:2 (6): 487-496
被引量:144
标识
DOI:10.1007/s13238-011-1061-y
摘要
An increasing body of evidence shows that the lipid droplet, a neutral lipid storage organelle, plays a role in lipid metabolism and energy homeostasis through its interaction with mitochondria. However, the cellular functions and molecular mechanisms of the interaction remain ambiguous. Here we present data from transmission electron microscopy, fluorescence imaging, and reconstitution assays, demonstrating that lipid droplets physically contact mitochondria in vivo and in vitro. Using a bimolecular fluorescence complementation assay in Saccharomyces cerevisiae, we generated an interactomic map of protein-protein contacts of lipid droplets with mitochondria and peroxisomes. The lipid droplet proteins Erg6 and Pet10 were found to be involved in 75% of the interactions detected. Interestingly, interactions between 3 pairs of lipid metabolic enzymes were detected. Collectively, these data demonstrate that lipid droplets make physical contacts with mitochondria and peroxisomes, and reveal specific molecular interactions that suggest active participation of lipid droplets in lipid metabolism in yeast.
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