Chlorella is a microalga that has been widely used in medicine, agriculture and environment. At present, there has been a founded equipped cultural pond to mass-produce of this alga all over the world. In this study, the effect of extracted salt from Urmia Lake with concentrations of 10, 30 and 50 g. L-1 on Chlorella sp. Biological parameters were investigated. The results indicated that in all treatments, the highest number of cells was observed on the 20th day while there were no significant differences among various salt concentrations. The highest and the lowest chlorophyll a values were reported in 50 g.L-1 and 30 g.L-1 salinities respectively although, the highest chlorophyll a value was obtained from 50 g.L-1 salinity on the 5th day. This study indicated that 50 g. L-1 salinity produced the highest Beta-carotene on days 10 and 15.