Regenerating myofiber directs Tregs and Th17 responses in inflamed muscle through the intrinsic TGF-β signaling-mediated IL-6 production

炎症 骨骼肌 心肌细胞 细胞生物学 细胞因子 肌发生 转化生长因子 生物 信号转导 免疫系统 免疫学 内科学 内分泌学 医学
作者
Tao Huang,Jingwen Huang,Zhaohong Liao,Haiqiang Lan,Xiaoting Jian,Ruicai Gu,Jun Ouyang,Jijie Hu,Hua Liao
出处
期刊:American Journal of Physiology-endocrinology and Metabolism [American Physiological Society]
卷期号:323 (1): E92-E106 被引量:6
标识
DOI:10.1152/ajpendo.00247.2021
摘要

Transforming growth factor-β (TGF-β) is considered to be an important immune regulatory cytokine. However, it remains unknown whether and how the muscle fiber specific-TGF-β signaling is directly involved in intramuscular inflammatory regulation by affecting T cells. Here, we addressed these in a mouse tibialis anterior muscle Cardiotoxin injection-induced injury repair model in muscle creatine kinase (MCK)-Cre control or transgenic mice with TGF-β receptor II (TGF-βr2) being specifically deleted in muscle cells (SM TGF-βr2-/-). In control mice, TGF-β2 and TGF-βr2 were found significantly upregulated in muscle after the acute injury. In mutant mice, deficiency of TGF-β signaling in muscle cells caused more serious muscle inflammation, with the increased infiltration of macrophages and CD4+ T cells at the degeneration stage (D4) and the early stage of regeneration (D7) after myoinjury. Notably, the loss of TGF-β signaling in myofibers dramatically affected CD4+ T cell function and delayed T cells withdrawal at the later stage of muscle regeneration (D10 and D15), marked by the elevated Th17, but the impaired Tregs response. Furthermore, in vivo and in vitro, the intrinsic TGF-β signaling affected immune behaviors of muscle cells and directed CD4+ T cells differentiation by impairing IL-6 production and release. It suggests that local muscle inflammation can be inhibited potentially by directly activating the TGF-β signaling pathway in muscle cells to suppress Th17, but induce Tregs responses. Thus, according to the results of this study, we found a new idea for the control of local acute inflammation in skeletal muscle.NEW & NOTEWORTHY Myofiber mediates muscle inflammatory response through activating the intrinsic TGF-β signaling. The specific TGF-β signaling activation contributes to myofiber IL-6 production and directs muscle-specific Th17 and Treg cell responses.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
于芋菊完成签到,获得积分0
1秒前
coo完成签到,获得积分10
1秒前
1秒前
LIU完成签到,获得积分20
2秒前
顾矜应助新奇采纳,获得10
4秒前
hs发布了新的文献求助10
6秒前
二队淼队长完成签到,获得积分10
7秒前
7秒前
8秒前
无聊的完成签到,获得积分10
8秒前
诚c完成签到,获得积分10
8秒前
8秒前
11秒前
彳亍1117应助韭菜采纳,获得10
12秒前
wanci应助韭菜采纳,获得10
12秒前
Ch完成签到 ,获得积分10
13秒前
刘小二拌面完成签到,获得积分10
13秒前
静谧完成签到 ,获得积分10
14秒前
甘乐完成签到,获得积分10
15秒前
~~~发布了新的文献求助10
16秒前
醉熏的幼珊完成签到,获得积分10
17秒前
完美世界应助hs采纳,获得10
20秒前
21秒前
李爱国应助科研通管家采纳,获得10
21秒前
bkagyin应助科研通管家采纳,获得10
21秒前
21秒前
搜集达人应助科研通管家采纳,获得10
21秒前
FashionBoy应助科研通管家采纳,获得10
21秒前
无花果应助科研通管家采纳,获得10
21秒前
酷波er应助科研通管家采纳,获得10
21秒前
CodeCraft应助科研通管家采纳,获得10
21秒前
打打应助科研通管家采纳,获得10
21秒前
斯文败类应助科研通管家采纳,获得10
22秒前
科研通AI2S应助科研通管家采纳,获得10
22秒前
科研通AI2S应助科研通管家采纳,获得10
22秒前
CipherSage应助科研通管家采纳,获得10
22秒前
22秒前
22秒前
22秒前
星辰大海应助科研通管家采纳,获得10
22秒前
高分求助中
The Oxford Handbook of Social Cognition (Second Edition, 2024) 1050
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Handbook of Qualitative Cross-Cultural Research Methods 600
Chen Hansheng: China’s Last Romantic Revolutionary 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3139867
求助须知:如何正确求助?哪些是违规求助? 2790746
关于积分的说明 7796497
捐赠科研通 2447159
什么是DOI,文献DOI怎么找? 1301623
科研通“疑难数据库(出版商)”最低求助积分说明 626313
版权声明 601185