亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Integrated 16S rRNA gene sequencing and LC/MS-based metabolomics ascertained synergistic influences of the combination of acupuncture and NaoMaiTong on ischemic stroke

针灸科 医学 中医药 冲程(发动机) 神经营养因子 代谢组学 药理学 缺血性中风 神经学 生物信息学 治疗效果 传统医学 内科学 缺血 病理 生物 替代医学 受体 工程类 精神科 机械工程
作者
Minghua Xian,Lin Shen,Sikai Zhan,Shaoru Chen,Huiting Lin,Jiale Cai,Tao Hu,Shumei Wang
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:293: 115281-115281 被引量:26
标识
DOI:10.1016/j.jep.2022.115281
摘要

Acupuncture is an effective therapy for ischemic stroke, which has been widely used in China and gradually accepted in more countries and regions recently. In addition, Chinese medicine also plays an important role in stroke treatment, among which NaoMaiTong (NMT) is an example of an effective herbal formula for the treatment of stroke. A therapeutic strategy that combines acupuncture and medicine was widely used in stroke patients. However, the synergistic influences and mechanisms of combined acupuncture and medicine on ischemic stroke have not yet been entirely elucidated.The purpose of this study is to explore whether acupuncture and medicine combination treatments can produce synergism by using NMT, a clinically effective Chinese medicinal formula for the treatment of ischemic stroke for decades and has been demonstrated to be effective against ischemic brain injury, as a probe. Meanwhile, the potential mechanisms were investigated via cecal microbiome and plasma metabolomics to provide more strategies and basis for acupuncture-medicine combination for stroke.Adopted middle-cerebral artery occlusion/reperfusion (MCAO/R) rat models, the effect for the stroke of the combination treatment consisting of acupuncture and NMT was evaluated by detecting neurological issues, cerebral infarct dimensions, levels of inflammatory factors (IL-6, IL-1β, TNF-α) and oxidative stress factors (SOD, MDA) and brain-derived neurotrophic factor (BDNF). Subsequently,16S rRNA gene sequencing and LC/MS-based metabolomic analysis were utilized to explore the characteristics of cecal-contents microecology and plasma metabolic profile, respectively. Finally, the correlation between intestinal microecological characteristics and plasma metabolic characteristics was analyzed to explore the potential mechanism of the acupuncture-NMT combination.The efficacy of acupuncture-NMT therapy was more effective than a single treatment on ischemic stroke, with more effectively reduced infarct sizes, improved neurobehavioral deficits, and alleviated oxidative stress and inflammatory responses. Besides, the combination therapy not only adjusted gut microbiota disturbances by enriching species diversity, reducing the abundance of pathogenic bacteria (such as Escherichia-Shaigella), as well as increasing the abundance of beneficial bacteria (such as Turicibacter, Bifidobacterium), but also improved metabolic disorders by reversing metabolite plasma levels to normality. The results of the correlation analysis demonstrated a significant association between intestinal microbiota and plasma metabolic profile, especially the strong correlation of Turicibacter and isoflavones phyto-estrogens metabolites.The combination of acupuncture and NMT could produce synergism, suggesting acupuncture-medicine combination therapy might be more conducive to the recovery of ischemic stroke. And the potential mechanism was probably related to the mediation of intestinal microecology and plasma metabolism. Turicibacter and isoflavones phyto-estrogens metabolites might be the targets for acupuncture-NMT combination for stroke. Our current findings could provide a potential therapeutic strategy against ischemic stroke.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
某个不想做人的dio完成签到 ,获得积分10
1秒前
冷静新烟发布了新的文献求助10
3秒前
Eden完成签到,获得积分10
4秒前
13秒前
烟花应助灵感大王喵采纳,获得200
14秒前
夏夏完成签到 ,获得积分10
17秒前
21秒前
22秒前
潘善若发布了新的文献求助10
26秒前
田様应助El采纳,获得10
28秒前
隔壁巷子里的劉完成签到 ,获得积分10
31秒前
李爱国应助潘善若采纳,获得10
35秒前
42秒前
五十一完成签到 ,获得积分10
50秒前
奇遇完成签到 ,获得积分10
51秒前
1分钟前
含糊的安柏完成签到,获得积分10
1分钟前
huihongzeng发布了新的文献求助10
1分钟前
NexusExplorer应助含糊的安柏采纳,获得10
1分钟前
1分钟前
冷静新烟完成签到,获得积分20
1分钟前
冷静新烟发布了新的文献求助10
1分钟前
简单山水发布了新的文献求助10
1分钟前
Novajet完成签到,获得积分10
1分钟前
yx_cheng应助归海浩阑采纳,获得30
1分钟前
ding应助科研通管家采纳,获得10
1分钟前
1分钟前
大碗完成签到 ,获得积分10
1分钟前
桐桐应助简单山水采纳,获得10
1分钟前
1分钟前
Novajet发布了新的文献求助10
1分钟前
小碗完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
黄桂斌完成签到,获得积分10
1分钟前
许三问完成签到 ,获得积分0
1分钟前
略略完成签到,获得积分10
1分钟前
情怀应助Novajet采纳,获得10
1分钟前
小凯完成签到 ,获得积分10
1分钟前
乐乐乐乐乐乐应助Yang采纳,获得10
1分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990012
求助须知:如何正确求助?哪些是违规求助? 3532077
关于积分的说明 11256227
捐赠科研通 3270933
什么是DOI,文献DOI怎么找? 1805139
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228