Protective Effects of Manuka Honey Against Diazinon Induced Hepatorenal Toxicity in Adult Male Rats

Manuka honey is mono-floral honey produced by Manuka myrtle tree.MH has anti-inflammatory, anti-bacterial, anticancer, wound healing and other benefits.This experimental study aimed to monitor Diazinon administration effects on liver and kidney of adult male Albino rats and protective value of 6 weeks administration of MH against effects of Diazinon and mechanism of their actions.Forty rats weighing 180-220 g divided into four groups (10 rats each): Group I (control group): rats received 0.2 mg/kg corn oil, daily for 6 weeks.Group II (DZN group): rats orally administrated 50 mg/kg DZN in corn oil, daily for 6 weeks.Group III (MH group): rats orally administrated 1.5 g/kg MH daily for 6 weeks.Group IV (DZN + MH group): rats orally administrated 50 mg/kg DZN and 1.5 g/kg MH, daily for 6 weeks.After six weeks, blood samples collected from retro-orbital venous plexus.Serum samples used to determine levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), total bilirubin, creatinine, blood urea nitrogen (BUN), total protein (TP), albumin (ALB), glucose, triglycerides (TG), total cholesterol (CHO), high-density lipoprotein cholesterol (HDL-c), low-density lipoprotein cholesterol (LDL-c) very-lowdensity lipoprotein cholesterol (VLDL-c), creatinine kinase (CK) and lactate dehydrogenase (LDH).After blood sampling, livers and kidneys isolated, fixed in 10% buffered formalin and examined under light microscope.DZN led to hyperglycemia, increased in serum levels of triglyceride, CHO, LDL-C, VLDL-C, AST, ALT, ALP, GGT, creatinine, Blood urea nitrogen, CK and LDH but significant decreased in total proteins and albumin.Administration of MH led to improvement of blood glucose, lipid profile, liver function tests and kidney function tests, Tissue destructive enzymes.These improvements were confirmed histologically by examination of hepatic and renal tissues.In conclusions, DZN had hepatotoxicity and renal toxicity that improved by Manuka honey administration for 6 weeks.