Development of Design Rules for Reliable Antisense RNA Behavior in E. coli

合成生物学 合理设计 计算生物学 核糖核酸 反义RNA 生物 计算机科学 正交性 遗传学 生化工程 基因 工程类 数学 几何学
作者
Allison Hoynes-O’Connor,Tae Seok Moon
出处
期刊:ACS Synthetic Biology [American Chemical Society]
卷期号:5 (12): 1441-1454 被引量:55
标识
DOI:10.1021/acssynbio.6b00036
摘要

A key driver of synthetic biology is the development of designable genetic parts with predictable behaviors that can be quickly implemented in complex genetic systems. However, the intrinsic complexity of gene regulation can make the rational design of genetic parts challenging. This challenge is apparent in the design of antisense RNA (asRNA) regulators. Though asRNAs are well-known regulators, the literature governing their design is conflicting and leaves the synthetic biology community without clear asRNA design rules. The goal of this study is to perform a comprehensive experimental characterization and statistical analysis of 121 unique asRNA regulators in order to resolve the conflicts that currently exist in the literature. asRNAs usually consist of two regions, the Hfq binding site and the target binding region (TBR). First, the behaviors of several high-performing Hfq binding sites were compared, in terms of their ability to improve repression efficiencies and their orthogonality. Next, a large-scale analysis of TBR design parameters identified asRNA length, the thermodynamics of asRNA–mRNA complex formation, and the percent of target mismatch as key parameters for TBR design. These parameters were used to develop simple asRNA design rules. Finally, these design rules were applied to construct both a simple and a complex genetic circuit containing different asRNAs, and predictable behavior was observed in both circuits. The results presented in this study will drive synthetic biology forward by providing useful design guidelines for the construction of asRNA regulators with predictable behaviors.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
3秒前
亮亮完成签到,获得积分10
4秒前
6秒前
6秒前
刘禹彤完成签到,获得积分10
7秒前
Mia发布了新的文献求助10
8秒前
8秒前
11秒前
11秒前
Tsing发布了新的文献求助10
11秒前
奋斗的孤风完成签到,获得积分10
12秒前
12秒前
13秒前
吃鱼完成签到 ,获得积分10
13秒前
LF-Scie完成签到,获得积分10
13秒前
小超超完成签到 ,获得积分10
15秒前
CA发布了新的文献求助10
15秒前
yewungs发布了新的文献求助30
16秒前
16秒前
打打应助Chelry采纳,获得10
16秒前
于友卉发布了新的文献求助10
17秒前
19秒前
乐乐应助小秋秋采纳,获得10
20秒前
切咖啡完成签到 ,获得积分20
22秒前
WMQkingofk发布了新的文献求助10
22秒前
科目三应助细腻老四采纳,获得10
22秒前
23秒前
26秒前
长情语蕊发布了新的文献求助10
28秒前
29秒前
coc完成签到,获得积分10
30秒前
makabaka发布了新的文献求助10
30秒前
30秒前
31秒前
Cu完成签到 ,获得积分10
31秒前
32秒前
赵纤完成签到,获得积分10
33秒前
makabaka完成签到,获得积分10
35秒前
细腻老四发布了新的文献求助10
35秒前
英俊的铭应助科研通管家采纳,获得10
35秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Indomethacinのヒトにおける経皮吸収 400
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 370
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
Aktuelle Entwicklungen in der linguistischen Forschung 300
Current Perspectives on Generative SLA - Processing, Influence, and Interfaces 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3991883
求助须知:如何正确求助?哪些是违规求助? 3533014
关于积分的说明 11260344
捐赠科研通 3272297
什么是DOI,文献DOI怎么找? 1805688
邀请新用户注册赠送积分活动 882609
科研通“疑难数据库(出版商)”最低求助积分说明 809425