Abstract 3438: DNA methylation profiles of Helicobacter pylori strains from patients with gastric cancer and gastritis

DNA甲基化 甲基化 生物 甲基转移酶 基因组 遗传学 幽门螺杆菌 DNA 基因 CpG站点 基因表达
作者
M. Constanza Camargo,Javier Torres,Jay V. Solnick,Castle Raley,Dylan Storey,Marina Becker,Roberto C. Torres,Allison M. Weis,Carol Huang,Nguyet Kong,Lori M. Hanson,Yongmei Zhao,Xiongfong Chen,Bao Tran,Bart C. Weimer,Charles S. Rabkin
出处
期刊:Cancer Research [American Association for Cancer Research]
卷期号:76 (14_Supplement): 3438-3438
标识
DOI:10.1158/1538-7445.am2016-3438
摘要

Abstract BACKGROUND: Helicobacter pylori, a gram-negative bacterium associated with a spectrum of benign and malignant gastric conditions, is one of the most genetically variable pathogens. Its genome encodes a large number of DNA methyltransferases targeting specific motif sequences of ∼4-15 bp. DNA base modifications epigenetically regulate gene expression and genome-wide methylation profiles (methylomes) have been hypothesized to be associated with virulence. A limited number of H. pylori methylomes have been published to date, precluding meaningful analyses of clinical correlates. METHODS: We sequenced 30 H. pylori clinical isolates from Mexico City using Pacific Biosciences’ Single Molecule Real-Time (SMRT) technology, a unique platform that simultaneously determines a wide range of DNA base modifications. The resulting methylome data were compared for the 15 strains from gastric cancer cases vs. the 15 from non-atrophic gastritis controls. RESULTS: All 30 strains exhibited high levels of DNA methylation throughout their genomes. Three types of base modification were detected: N6-methyladenine (m6A), N4-methylcytosine (m4C) and 5-methylcytosine (m5C). m6A was more common than m4C, and m5C was rare. Over 100 different motifs were detected, including many novel motifs not found in the three reference strains. For most methylated motifs, >95% of all occurrences in a given genome were methylated. Individual strains had between 14 and 28 different motifs. Some of the methylated motifs were conserved across all (e.g., Cm6ATG) or almost all (e.g., Gm6ACT) strains, but the majority were found in only one or two strains. Two conserved motifs, GTm6AC and GTNNm6AC, were significantly (P<0.05) more common in cancer cases than controls. CONCLUSIONS: The H. pylori methylome varies widely across strains, in concert with the high plasticity of its genome. Epigenetic modifications may modulate bacterial pathogenicity. These intriguing observations warrant replication with larger numbers and multiple populations in different geographic areas. The inferred novel methyltransferases remain to be identified and functionally characterized. This report greatly increases the number of H. pylori strains with defined methylomes, and represents the first effort to associate these features with clinical outcome. Availability of these genomic and epigenomic data will enhance understanding of H. pylori pathogenesis. Citation Format: M. Constanza Camargo, Javier Torres, Jay V. Solnick, Castle Raley, Dylan Storey, Marina Becker, Roberto Torres, Allison Weis, Carol Huang, Nguyet Kong, Lori M. Hanson, Yongmei Zhao, Xiongfong Chen, Bao Tran, Bart Weimer, Charles S. Rabkin. DNA methylation profiles of Helicobacter pylori strains from patients with gastric cancer and gastritis. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 3438.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
qwe1108完成签到 ,获得积分10
刚刚
1秒前
jane完成签到 ,获得积分10
4秒前
6秒前
瑾玉完成签到,获得积分10
6秒前
8秒前
Akim应助duckspy采纳,获得10
8秒前
那种完成签到,获得积分10
8秒前
liuyanq完成签到,获得积分20
8秒前
9秒前
普鲁卡因发布了新的文献求助10
10秒前
加油杨完成签到 ,获得积分10
11秒前
liuyanq发布了新的文献求助10
14秒前
随风完成签到,获得积分10
17秒前
量子星尘发布了新的文献求助10
19秒前
米九完成签到,获得积分10
21秒前
zhao完成签到,获得积分10
24秒前
普鲁卡因发布了新的文献求助10
24秒前
zj完成签到,获得积分10
30秒前
蓝橙完成签到,获得积分10
31秒前
35秒前
GD88完成签到,获得积分10
36秒前
糟糕的梨愁完成签到,获得积分10
37秒前
莫西莫西完成签到 ,获得积分10
38秒前
小趴蔡完成签到 ,获得积分10
40秒前
唐唐发布了新的文献求助10
40秒前
飘逸剑身完成签到,获得积分10
43秒前
airtermis完成签到 ,获得积分10
43秒前
gfasdjsjdsjd完成签到,获得积分10
44秒前
44秒前
杨宁完成签到 ,获得积分10
44秒前
MchemG应助transition采纳,获得20
45秒前
科研通AI2S应助科研通管家采纳,获得10
46秒前
lxy发布了新的文献求助10
48秒前
gfasdjsjdsjd发布了新的文献求助10
49秒前
JCao727完成签到,获得积分10
49秒前
49秒前
50秒前
OAHCIL完成签到 ,获得积分10
51秒前
lixueao发布了新的文献求助10
52秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038128
求助须知:如何正确求助?哪些是违规求助? 3575831
关于积分的说明 11373827
捐赠科研通 3305610
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022