转染
上皮-间质转换
小RNA
异位表达
污渍
分子生物学
细胞培养
波形蛋白
过渡(遗传学)
细胞生物学
钙粘蛋白
基因敲除
连环素
下调和上调
化学
生物
信使核糖核酸
免疫组织化学
细胞
基因
信号转导
Wnt信号通路
免疫学
遗传学
作者
Luo-Yuan Cao,Jing Yang,Xianguo Fu,Lin Yinghua,Feng Lin,Baoying Huang
出处
期刊:PubMed
日期:2016-12-20
卷期号:36 (12): 1700-1705
被引量:4
摘要
To explore the role of miR-205 in regulating epithelial-messenchymal transition (EMT) in proximal tubular cell line HK-2 cells and the underlying mechanism.HK-2 cells transfected with miR-205 mimics or a scrambled control sequence were examined for miR-205 expressions and mRNA levels of ZEB1, E-cadherin, and α-SMA using real-time qPCR; the protein levels of ZEB1, ZEB2, E-cadherin, and α-SMA were detected with Western blotting. Immunohistochemistry was performed to examine the ectopic expression of β-catenin and E-cadherin expression in the cells.The expression levels of ZEB1 and ZEB2 decreased significantly (P<0.01) while E-cadherin expression was up-regulated (P<0.01) in cells transfected with miR-205 mimics. Transfection with miR-205 mimics also markedly down-regulated the expression of α-SMA (P<0.01), a marker of mesenchymal cells that play an important role in EMT of HK-2 cells. The ectopic expression of β-catenin was inhibited by miR-205 mimics in HK-2 cells.miR-205 inhibits EMT in HK-2 cells by down-regulating the expression levels of ZEB1 and ZEB2.
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