Dual Recognition Strategy for Specific and Sensitive Detection of Bacteria Using Aptamer-Coated Magnetic Beads and Antibiotic-Capped Gold Nanoclusters

化学 纳米团簇 适体 细菌 抗生素 纳米技术 磁选 组合化学 生物化学 分子生物学 有机化学 冶金 遗传学 材料科学 生物
作者
Dan Cheng,Mengqun Yu,Fei Fu,Weiye Han,Li Gan,Jianping Xie,Yang Song,Mark T. Swihart,Erqun Song
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:88 (1): 820-825 被引量:168
标识
DOI:10.1021/acs.analchem.5b03320
摘要

Food poisoning and infectious diseases caused by pathogenic bacteria such as Staphylococcus aureus (SA) are serious public health concerns. A method of specific, sensitive, and rapid detection of such bacteria is essential and important. This study presents a strategy that combines aptamer and antibiotic-based dual recognition units with magnetic enrichment and fluorescent detection to achieve specific and sensitive quantification of SA in authentic specimens and in the presence of much higher concentrations of other bacteria. Aptamer-coated magnetic beads (Apt-MB) were employed for specific capture of SA. Vancomycin-stabilized fluorescent gold nanoclusters (AuNCs@Van) were prepared by a simple one-step process and used for sensitive quantification of SA in the range of 32–108 cfu/mL with the detection limit of 16 cfu/mL via a fluorescence intensity measurement. And using this strategy, about 70 cfu/mL of SA in complex samples (containing 3 × 108 cfu/mL of other different contaminated bacteria) could be successfully detected. In comparison to prior studies, the developed strategy here not only simplifies the preparation procedure of the fluorescent probes (AuNCs@Van) to a great extent but also could sensitively quantify SA in the presence of much higher concentrations of other bacteria directly with good accuracy. Moreover, the aptamer and antibiotic used in this strategy are much less expensive and widely available compared to common-used antibodies, making it cost-effective. This general aptamer- and antibiotic-based dual recognition strategy, combined with magnetic enrichment and fluorescent detection of trace bacteria, shows great potential application in monitoring bacterial food contamination and infectious diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
RESLR驳回了孙燕应助
1秒前
ysy发布了新的文献求助10
2秒前
小二郎应助momo采纳,获得10
2秒前
zzrs完成签到,获得积分10
2秒前
量子星尘发布了新的文献求助10
2秒前
陈红安发布了新的文献求助30
3秒前
3秒前
yyy完成签到,获得积分10
5秒前
小小aa16完成签到,获得积分10
5秒前
小呆荣发布了新的文献求助10
6秒前
6秒前
7秒前
7秒前
8秒前
幽默平安发布了新的文献求助10
8秒前
奋斗夏烟发布了新的文献求助10
10秒前
12秒前
ylh完成签到,获得积分10
14秒前
Owen应助幽默平安采纳,获得10
14秒前
乖猫要努力应助YJ888采纳,获得10
15秒前
15秒前
丘比特应助穆羊青采纳,获得10
16秒前
sasa完成签到,获得积分10
16秒前
sadascaqwqw发布了新的文献求助10
17秒前
17秒前
18秒前
18秒前
小宇子发布了新的文献求助10
19秒前
19秒前
锤子发布了新的文献求助25
20秒前
奋斗夏烟完成签到,获得积分10
20秒前
20秒前
zzrs发布了新的文献求助10
20秒前
Mo发布了新的文献求助10
22秒前
老大蒂亚戈应助YJ888采纳,获得10
22秒前
欣慰的水瑶完成签到,获得积分10
23秒前
momo发布了新的文献求助10
23秒前
25秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989334
求助须知:如何正确求助?哪些是违规求助? 3531428
关于积分的说明 11253936
捐赠科研通 3270119
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809173