酪氨酸
DNA连接酶
生物化学
微管蛋白
生物正交化学
泛素连接酶
生物
化学
蛋白质工程
细胞生物学
分子生物学
泛素
酶
组合化学
微管
点击化学
基因
作者
Dominik Schumacher,Jonas Helma,Florian A. Mann,Garwin Pichler,Francesco Natale,Eberhard Krause,M. Cristina Cardoso,Christian P. R. Hackenberger,Heinrich Leonhardt
标识
DOI:10.1002/anie.201505456
摘要
Abstract A novel chemoenzymatic approach for simple and fast site‐specific protein labeling is reported. Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles to proteins containing a short tubulin‐derived recognition sequence (Tub‐tag). This novel strategy enables a broad range of high‐yielding and fast chemoselective C‐terminal protein modifications on isolated proteins or in cell lysates for applications in biochemistry, cell biology, and beyond, as demonstrated by the site‐specific labeling of nanobodies, GFP, and ubiquitin.
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