Antibacterial activity of chlorhexidine after final irrigation with ethanol: CLSM and culture‐based method analysis

洗必泰 粪肠球菌 生物膜 化学 生理盐水 乙醇 菌落形成单位 共焦激光扫描显微镜 微生物学 抗菌活性 牙本质 牙科 细菌 生物 大肠杆菌 医学 生物化学 遗传学 基因 内分泌学
作者
Juliana Martins Suman,Milton Carlos Kuga,Ricardo Abreu da Rosa,Manuela Favarin Santini,Renata Grazziotin‐Soares,Francisco Montagner,Marcus Vinícius Reis Só
出处
期刊:Microscopy Research and Technique [Wiley]
卷期号:78 (8): 682-687 被引量:7
标识
DOI:10.1002/jemt.22525
摘要

This study investigated the effect of 95% ethanol on the antibacterial properties of 2% chlorexidine (CHX) over monospecies biofilm (Enterococcus faecalis) through a culture-based method, and over multispecies biofilm using confocal laser scanning microscopy (CLSM). For monospecies model, E. faecalis biofilm was induced in 40 root canals. The irrigation procedures were: S-saline solution; S/CHX-saline solution + CHX; E-ethanol; and E/CHX-ethanol + CHX. Microbial sampling was performed at three periods: before (S1), immediately after (S2), and 72 h after the final flush (S3). For multispecies biofilm model, 28 sterilized bovine dentin blocks were fixed on a removable orthodontic device to allow intraoral biofilm development. Seven samples were used in each group. Statistical analysis was carried out by using the Kruskal-Wallis test and Dunn's test for multiple comparisons. There was a significant reduction in CFUs count immediately after the final flush (S2) in all experimental groups (P < 0.05). However, only S/CHX, E and E/CHX groups had CFU counts close to zero, without differences among them (P > 0.05). After 72h (S3), the S/CHX and E/CHX groups had CFU counts near zero (P > 0.05). The CFU count increased in S3 for S and E groups (P < 0.05). CLSM showed that the percentages of remaining live cells were similar in S/CHX, E, and E/CHX groups (P > 0.05). The S group had the highest percentage of live cells (P < 0.05). The 95% ethanol did not interfere in the antibacterial properties of 2% CHX over mono- and multispecies biofilms.
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