A minimal genome design to maximally guarantee fertile inter-subspecific hybrid rice

生物 基因组 生物技术 遗传学 计算生物学 进化生物学 基因
作者
Penghui Zhou,Zhengji Wang,Xingchen Zhu,Yao Tang,Liang Ye,Huihui Yu,Yating Li,Ningke Zhang,Ting Liu,Tian Wang,Yuying Wu,Dengyun Cao,Yuan Chen,Li Xu,Qinglu Zhang,Jinghua Xiao,Sibin Yu,Qifa Zhang,Jiaming Mi,Yidan Ouyang
出处
期刊:Molecular Plant [Elsevier BV]
卷期号:16 (4): 726-738 被引量:16
标识
DOI:10.1016/j.molp.2023.02.009
摘要

Hybrid rice has made considerable contributions to achieve the ambitious goal of food security for the world's population. Hybrid rice from indica/xian and japonica/geng subspecies shows much higher heterosis and is thereby an important innovation in promoting rice production in the next decade. However, such inter-subspecific hybrid rice has long suffered from serious hybrid sterility, which is a major challenge that needs to be addressed. In this study, we performed a genome design strategy to produce fertile inter-subspecific hybrid by creation of wide compatibility varieties that are able to overcome hybrid sterility. Based on combined genetic analyses in two indica-japonica crosses, we determined that four hybrid sterility loci, S5, f5, pf12 and Sc, are the major QTLs controlling inter-subspecific hybrid sterility and thus the minimal targets that can be manipulated for breeding sub-specific hybrid rice. We then cloned the pf12 locus, one of the most effective loci for hybrid male sterility, by map-based cloning, and showed that artificial disruption of pf12A gene at this locus could successfully rescue hybrid fertility. We further dissected the genetic basis of wide compatibility using three pairwise crosses from a wide-compatibility variety Dular and representative indica and japonica varieties. On this basis, we constructed and assembled different combinations of naturally compatible alleles of four loci, S5, Sc, pf12, and f5, and found that the improved lines could fully recover pollen and embryo sac fertility in test-crossed F1s, thereby completely fulfilling the demands of inter-subspecific hybrid spikelet fertility in agricultural production. This breeding scheme would facilitate redesign of future inter-subspecific hybrid rice with a higher yield potential.
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