辐照
体外
体外冲击波疗法
体内
活力测定
医学
后肢
放射治疗
纤维化
核医学
化学
男科
病理
细胞
内科学
外科
生物
生物技术
核物理学
物理
生物化学
作者
Sang Woo Park,Jaebong Shin,Bae Kwon Jeong,Sangjun Byun,Kyung Suk Lee,Jaehoon Choi
标识
DOI:10.1097/prs.0000000000011782
摘要
Background: Although radiation-induced skin injuries are a concern in patients receiving radiation therapy, there are few effective treatments. The aim of this study was to evaluate the protective effects of extracorporeal shock wave therapy (ESWT) on irradiated fibroblasts and mouse skin. Methods: In the in vitro study of human dermal fibroblasts, the experimental group was subjected to ESWT following irradiation (20 Gy). The control groups were only irradiated or only subjected to ESWT. At 24 or 48 h post-ESWT, cell viability, cell migration, and mRNA and protein expression were measured. In the in vivo study, the experimental group (7 mice) was treated with ESWT following irradiation (45 Gy). The control group (7 mice) was only irradiated. At 8 weeks post-irradiation, dorsal skin was harvested for histopathologic examination and protein isolation. Results: In dermal fibroblasts, treatment with ESWT increased viability of irradiated cells compared with irradiated-only and untreated cells ( p = 0.005). ESWT increased cell migration 24 h post-irradiation ( p = 0.002), and decreased TGF-β1 protein expression 48 h post-irradiation ( p = 0.024). In mice, ESWT decreased the level of radiation-related skin injury ( p = 0.006). Treatment of irradiated skin with ESWT decreased TGF-β1 ( p = 0.009) and phospho-Smad3 ( p = 0.009) protein expression, as well as decreasing myofibroblasts ( p = 0.047) and increasing vessel density ( p < 0.001). Conclusions: Our study demonstrated that ESWT alleviated radiation-induced fibrosis by downregulating TGF-β1 expression. It suggests the potential of ESWT for the treatment of radiation-induced fibrosis.
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